FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_70_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_70_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17999485
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG159600.014.7187215
GTATAGA128950.014.0001391
GTATTAG139700.013.80509951
GTATAAG121200.013.6989471
GTATATA296200.013.6340381
GTATAGG84150.013.6131091
GTATTAA180650.012.9232651
TATACAG165900.012.3242555
ATTATAC172150.011.91890053
TCTATAC127800.011.8001973
GTGTATA184500.011.7104661
GTATATG180700.011.6357461
GTATTAT215800.011.5574321
GTCCTAG82900.011.45700551
TATATAC289750.011.3853053
GTCTTAG104000.011.29369451
GTATAAT184850.011.2568471
CCTACAC99450.011.2272313
ACTGATC136450.011.2111458
CTATACT124900.011.2034774
Produced by FastQC (version 0.11.5)