FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18524496
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA122850.016.2884541
TATACTG175050.015.9045345
GTATAGG80350.015.8808151
GTATATA327450.015.1888571
GTATTAG134300.015.0077221
TATACAG173300.014.5171975
GTATAAG122000.013.7871381
CTATACT132850.013.2070454
CCTATAC76600.012.9671233
GTATTAT208500.012.9007171
GTGTAGG98200.012.8464671
TCTATAC122600.012.7736523
GTGTATA228550.012.4985891
TACACCG20600.012.3183545
GTATATG198200.012.2908411
CTAATAC130100.012.2601913
ATACTGT234200.011.8566966
TAATACT188750.011.830844
TATATAC330300.011.6556843
GTATTAA180300.011.5809111
Produced by FastQC (version 0.11.5)