FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973383
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2953.8742473E-722.118591
GTATATA11450.018.3623981
GGACCGT3252.202921E-517.846116
TTAGGGC3655.959711E-515.890373
GAACCGC4752.1470332E-615.2631196
GTGTATA7654.1836756E-1115.1633611
TATATAG7253.0377123E-1014.9999632
TAGTAGT4852.6662692E-614.9484184
GTATAAG5857.629569E-814.8717581
TAGGGCT4401.7615022E-514.8295094
GTGTAAG5506.160717E-714.4999651
CTAGACT4552.4173836E-514.3406234
GCCCTAA3558.6892204E-414.2957391
GTATTAT10800.014.0971861
GTATGTA6201.5530532E-714.0322231
GTATTAG7254.8930815E-913.9999651
GTATAGA7254.8930815E-913.9999651
GGAACCG4753.62524E-513.7368075
GTCCTAA3700.001188036213.7161841
GCCTAGA4252.1773209E-413.6470251
Produced by FastQC (version 0.11.5)