FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCATTAGTCTTATTGTGACACTTTTTTATATTATAAAAAGCAATCCAC11.9607843137254901No Hit
ATGGTTAAGTATCTATTCCGTGATTACTTTTCTTAGGAATGTGAGAAGAT11.9607843137254901No Hit
CGCATGGCCTGCAATACACGAGCCTTTACTCGACTGTCAGGGCATCGCAC11.9607843137254901No Hit
CAGTTGATGCAAAGTCTTTTTTTTGTTCGATTTTACATACGAAAAACACT11.9607843137254901No Hit
ATATGAAACTATATTGGTATATAAATACAAAGGTCAATTATTTAAGAGGT11.9607843137254901No Hit
CCATATTACTGTAATTAATATGAAAACCACAAAGATTTTATATCAAACAC11.9607843137254901No Hit
GCCGTCGGGTTCTTTGCTTTGCTAACGAAAGGGTAGGTTAGCTATTTGAT11.9607843137254901No Hit
ATTTAATATTTTATACTATATTCATTAAAAACAAAAAATATTGCAAGATC11.9607843137254901No Hit
TCTGTAGATATGTTAGAGTAAGATATTAACGTGTCCAAAGAAATACTCAA11.9607843137254901No Hit
CTATTAGGAATTCCAAGTTGGCCAAAATATCTAGATGATGACATCTTTGC11.9607843137254901No Hit
CAATGGTTCTCTAACTTCCATAGCATAGCTTTAAAAAAAACAAAGTTCCG11.9607843137254901No Hit
TTAATGTAGGGTTCAGTAGACTCAGGAAGGTTTATATGTTGCAAGACAGG11.9607843137254901No Hit
GACAATTTTATTTGTATATAATGGTTCGAAACCAACCAAATTAATAAAAA11.9607843137254901No Hit
CATAAAATTTAATATCCTTGAATAATCCGAAAATGTGAACTCATGTAAGC11.9607843137254901No Hit
GTCATGATACTTTATTTTCATTTTGGTAGGGTGTGGGTTTTTGAAGTTGT11.9607843137254901No Hit
CGGCAACATAATTGGGAGGCTGGGGTTGAAACGACATCAAGACTGGAGGT11.9607843137254901No Hit
GTGAGTGTGAAATGGGGGTCAGAATCACCCTAATTACAATTATTTGTGAC11.9607843137254901No Hit
CACCTTTGTTTTCAATGATAGTGACTGCATAATGTACACAAAAAAACTCA11.9607843137254901No Hit
TAGATATCGAGTTGTTCTATAACTTCATTGTCTTTGTTTTCCATTTTATG11.9607843137254901No Hit
TTTTAACATTGTATAAAAATTTAAACTGACAGATTGATGATTGAATTATG11.9607843137254901No Hit
GATTAGGACAGTTCAGGGTAAACTCACATGGTCGAACCAATTTCCTCACC11.9607843137254901No Hit
TTTTTTTGCATTCCTACATGGCGGAGTTAGCGGACCAAAAGACCACTTGT11.9607843137254901No Hit
GACTCAGTCTGTTAATTAATTCTCAAAGACACTAGCTGGACTGACTCTGA11.9607843137254901No Hit
CAACACTACAAACGAAAATTAAATAAACCATGTCCGCAACAGCAGAGAGA11.9607843137254901No Hit
CCTCGAAAATTGTTCAGCGTCTCCACCTACCATTTTTTTTCTTTTATCTA11.9607843137254901No Hit
ACGGTCTTGAATGTTCTTTGAAAACAAGATAGATGATTTGAAGTTTATTG11.9607843137254901No Hit
CCCGCTCCAAATTATTCATTGTTTCCCGAAGCCTTCTACCTAGGAAACTG11.9607843137254901No Hit
TTGTTATTCAATTGTGGATTAGCCTAGTACTTACATCTTCGTATAGGATG11.9607843137254901No Hit
GTGAATCCATGCGATCCTGTAAGTGGAAACTCGTTAACCGTAGCCACAAA11.9607843137254901No Hit
GAGGCAATGATAGCACTGTGAATGACGAACATCCCAGGAACGTCAGCAGC11.9607843137254901No Hit
CCCTCTATCTTATCTGCGCAATCTTACCAAAACAATTCGGATGAGTTGCT11.9607843137254901No Hit
GTGCTGTCCACGAGCGACAATAGGTCCTCTGTCTATGTTATAAAACGGTC11.9607843137254901No Hit
GAATAAATAAGTGGTTCAACAAGCTGTGTCTCAGAGTTTTAATTTTCACT11.9607843137254901No Hit
CAGTAATACTTACTCAATCTAAATAAAGAGAATCTAAAAGTCGGTACCAC11.9607843137254901No Hit
CACGACAATAACATCCAATTTTGGTTATTAATGTTTAATTTCCACTAATT11.9607843137254901No Hit
GTATAATTTTAACTTACAGTTCAATTTATAAACGATGTACTAAACTAATA11.9607843137254901No Hit
CTTCACCAAATTAACTAAATCGAAAGTCTTTGATCCTAAATTATGTTCAT11.9607843137254901No Hit
GACGATGTTACGTACCACTTAGCCAGCGCGACGCCTGCACATCTCATCTG11.9607843137254901No Hit
GGTACCGTCCCTGGACCTCGCCGGCCCGGCATTCCTCCCAAGGTGCCCAC11.9607843137254901No Hit
ATCGAATGGAACGGAATGAAATAGTCAATGAACTCGAATGGAATCATCAT11.9607843137254901No Hit
TATTTACACACTGATTTATATTGGCCACCATTAAGTATATACCCAATTTC11.9607843137254901No Hit
GAATTATTTTACCGACAATAAAAAAACCCCACCACCACACATACATCTTA11.9607843137254901No Hit
TATATATACATAAATTTTTAAAAATTTCCTAATGAAATTCCCGAAGACAA11.9607843137254901No Hit
AAAAATTAGTGTGTGTTTATGTTGAAGACCGCCACACCACCGTCCTCTTA11.9607843137254901No Hit
GTTTTACAAATAAGCATTAATAAGTCAAAATATCAAGTCAGTTTTAAGAG11.9607843137254901No Hit
CATCCATTCGATACCACACAAAACCAAAGCTTTCCTTACCCCTACTAAAC11.9607843137254901No Hit
GTTGGAAACAAAGAGCCTGAATGAGTTTATGTTATGAGAATAATTATTTT11.9607843137254901No Hit
CCGGACAGCGTCGCGGCGTGCCACGACCACGTCTCTTCTCTCACAGTGGT11.9607843137254901No Hit
TCTTAGCTCACTGAAACCTCTGCCTCTCAGGTTCAAGCAATTCTCAGCCT11.9607843137254901No Hit
GGTCGAACCACTAGACACCTTAACTTAATTTAAATTTAGATCAAATGAGT11.9607843137254901No Hit
AAAACAAAAACAAGAAAAAAATAAATAAACAAAATAAATTCACAAAAAAA11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCAC50.0145.05
TAGCTCA50.0145.04
CTTAGCT50.0145.02
CACTGAA50.0145.09
TCACTGA50.0145.08
TTAGCTC50.0145.03
TCTTAGC50.0145.01
TCAGCCT100.072.5145
CTCACTG100.072.57
GCTCACT100.072.56
Produced by FastQC (version 0.11.5)