FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_38_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_38_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16948084
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG118200.015.2117041
TATACTG149450.015.08731655
GTATAGG70250.014.6549961
GTATATA290300.014.6100781
GTATAGA115250.014.5316421
TATACAG160900.013.6081295
GTGTATA197950.013.55157851
CTAGTAC54850.013.0858973
CCTATAC69650.012.8035073
GTACTAG57950.012.7611681
TCTATAC103800.012.7121493
GTATTAT181500.012.6227551
GTATTAA163050.012.3169351
TAATACT162700.012.1207324
TATATAC287600.011.9490793
GTATAAG111700.011.9428591
ACTGATC135850.011.7943088
GTGTAAG123050.011.7250611
TACACTG159350.011.6020745
CTATACT114700.011.5673854
Produced by FastQC (version 0.11.5)