FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_32_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_32_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11631901
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG76800.016.9926621
GTATATA144400.016.7698611
GTATTAT99400.016.5573041
ATAATAC94550.014.7995273
GTATTAA95250.014.6907011
TATTATA129800.014.5228132
GTATAAG75100.014.4810991
TATACTG87550.014.4094375
TAATACT96050.014.1910464
GTATAGA71100.014.1740961
GTGTATA122850.014.1640111
TCTATAC64950.014.065123
GTATAAT92550.014.0225441
CTAATAC76700.013.5173863
ATTATAC88050.013.4217853
TATATAC147700.013.4009083
CACGAGC21950.013.2117605145
TATAATA134000.012.9854952
CTATACT68300.012.6322354
ATATACT113850.012.5454754
Produced by FastQC (version 0.11.5)