FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTCAAAGAACAACAGTTACTCACGTTAAGTTTGAAATGTGTCATCT11.9607843137254901No Hit
GTGGGAGGCTAGGCACTGAATGTGATTTGGGGCTTTCCAGGATCACAGAA11.9607843137254901No Hit
GATGGCAAGTGAGCAAAGTAGGTGGGCTTTGTGGGGTTTTATGTGCCTCT11.9607843137254901No Hit
CAAAAAGCAAAAATTATATTTAACACTTAATGGACAAAAATTTTAAAAAC11.9607843137254901No Hit
GAAAACCTGTCTGTACTAAAAATACAAAAATTAGCCAGGCATGATGGAAG11.9607843137254901No Hit
GGCTGGTCTCGAACTCCTGCCCTCAGGTGATCTGCCCGCCTAGGCCTCCC11.9607843137254901No Hit
TTAAAATTAATATCACTTATAAGAACATAAGATAAAAAATCGCAAGCAAG11.9607843137254901No Hit
TTAAAGGCGTGAGCCACACCACCAGGCCTATATTTGTAAGGTACGTTTTT11.9607843137254901No Hit
GTTTCTAATCTTCTTCTATTCTTGCATAGGTGTTAGATTATTTTGAGTAT11.9607843137254901No Hit
GGCTGCATGCAGTGCAGGTGCGTTGATGACTGCAGGCGGTGCTAAGCGTA11.9607843137254901No Hit
CAGCTCAAGGATGCTGATAAATCTGATGAGCTGCACATTGTCGCCCAAGA11.9607843137254901No Hit
CCCCAATACAGATACACAACACTCTAGTCGAACACAGAAAAAACCAATAA11.9607843137254901No Hit
ATAAATTTTGTATAATTTACATGATTGATTTAAAATTAAAAATAAGAACT11.9607843137254901No Hit
AAAAAGGACAAGAATTGACCACATTTTGAACCCCTTGCTATCAACACACT11.9607843137254901No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.9607843137254901No Hit
GAATCTATCTCCCAAATGATTAATAAACACAAAACAATACATTTAAATAG11.9607843137254901No Hit
GGGGTGTCTAACTGTGAGTACACAGGAAATGAGAGCAATAAGGTTATTAG11.9607843137254901No Hit
AAATAATAATGACCTCCATTGTAAATTAAATCGAGTGCAATTCACAAAGG11.9607843137254901No Hit
CCCCTATGGATGGCTTATTACAGATAATTGTATTTCTCACTGGTCGAAAT11.9607843137254901No Hit
GCTCTAAACCATACATATAAAAAAAGTTGTTGAAGAAGAACCATTCGTTC11.9607843137254901No Hit
CTTTTGATCTAATCTAATTACTATAATATTGTTATGCCTCTGTCATTTTG11.9607843137254901No Hit
GGGCCAGGTTTCGCTCCTAATATATCTTCACTACGATCTGAGGACTGAAG11.9607843137254901No Hit
GGAATGGAATCATTGAATGGACTCGAAAGGGATCATGATTGAATGGAATT11.9607843137254901No Hit
CTGCCGCGTTCTGAGAAACAACGGTGTGATGGGTGTCTTCACGCCTCAGA11.9607843137254901No Hit
ATAAAATTGATTTATTTAAATAGTATTCCATTCTAGTACCGGTCATTATT11.9607843137254901No Hit
CCTGAAATGAAATGATATGTATTAATCGTCCTCAAAAAGGTCTGGGAGTC11.9607843137254901No Hit
ACTTACCACTCTGAGTCATTTCAGTGCAGCATTTAGACAATTTACATTGA11.9607843137254901No Hit
CATGTATAGAGAGTATACATTTAATTAGATATATTATATTTAATCTTTTC11.9607843137254901No Hit
GGCTCTAATACCTTGCGTGATTTAGGGCCATGTTGTGTGCCTGTCGGACT11.9607843137254901No Hit
CATTCTGAGGTTCTGGATACACATTATCCTTAGGGGGCACACTATTCAAC11.9607843137254901No Hit
GAATGAACAAGTTACAACAAAAATTTATGAGCCATGAAGTGAAATAAGAA11.9607843137254901No Hit
CAATTAAAATGTACAGGTATAGAAAATTTGATTTCCGTACTAGTAGCATG11.9607843137254901No Hit
GAGATTGATAGATAGATAGATCAGCTAATGTCTGTTTCGCTCGTCAAGCC11.9607843137254901No Hit
GTCTTACCCGCTCCAAAACTATTAATCATTCGGTCATATATTAATAGATA11.9607843137254901No Hit
GTATTCAGGAGACCCATCTCAAGTGCAGAGCCATGAATAGGCTCAAAATA11.9607843137254901No Hit
GGCTCCGCACGTGGGTCCCCCGGCGTGCTCAGCATTAGTTGAGTCTCCAG11.9607843137254901No Hit
GGTAAAGACATGAAAATGCAAAACAATACTCTTGTATTTTTATCAATGAA11.9607843137254901No Hit
CAAATATAGTCTCTTGATGGTAATGGATAAATTTGACAAGAGAAATAATT11.9607843137254901No Hit
GCCCAGATATTTGGTTAAACATTATTTCTGGGTGTGTCTGTGAGGGTGTT11.9607843137254901No Hit
GTCTCACCGGGCCCCATGCCTAGACGGACCTTTCACATTGGGTACTGTGC11.9607843137254901No Hit
GGGAGTATCTGAAAAAACTAAGATATGTTATGCGTGGTGCAAAAATTAGA11.9607843137254901No Hit
TGTCTGACAAAAAAAGTGTAAGTAACAAATCACAGGAAATGGAACTTATT11.9607843137254901No Hit
TTGTAGTATAGTTTGAAGTGTGGTAGTGAGATGCCTCCAGCTTAGTTCTT11.9607843137254901No Hit
AGCCGGCTGTGTTGGGTTAATGTGTCAGTAATATTGCATCAAACAAACAC11.9607843137254901No Hit
GAATTGTATATATAAACTCTTCAAGTCTCTGGTTGACGCCTGACCCATGC11.9607843137254901No Hit
CAGCAACGAAATCCAATATCCAATTTCCTAGGCAACACAAAGAGGGGTTA11.9607843137254901No Hit
ATTAAACTCAGTCAGTATATGACGGGCAGGCTGAGACTAGGGAGAAGGAG11.9607843137254901No Hit
TTCAGATGCAGATACTTGAATTTCCTGGAATGTCTTACCGGGAGAGTTAC11.9607843137254901No Hit
GGTGGGTTATATAGAATGGTAGCTGTCCATTTATCCCTCACGACTGGAGA11.9607843137254901No Hit
GGCCCCGATGCTGAAGGGCCGTGACCCCATCGTAGATCACTGTCACCGAC11.9607843137254901No Hit
CCATGTACCTTTGTGTCTCCTTGCATTTAAAAATGCAGGTCATGATGTTT11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTT50.0145.05
CCATGTA50.0145.01
CCTTTGT50.0145.08
ACCTTTG50.0145.07
TACCTTT50.0145.06
CTTTGTG50.0145.09
ATGTACC50.0145.03
TGTACCT50.0145.04
CCAAGAA50.0145.0145
CATGTAC50.0145.02
Produced by FastQC (version 0.11.5)