FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_26_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_26_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10702648
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG8700.015.8334695
GTATAGG42600.015.3170331
GTATAAG67900.014.2011531
GTATTAG73900.013.7348951
TATACTG94800.013.6894645
GTATATA172500.013.6595381
GTATACG9056.148184E-1012.8177911
GTGTATA122150.012.3455831
GTATAGA69050.012.2846811
CTTACAC68150.012.2341493
GTACTAG34800.012.0834371
GTGTAGG52500.012.0143891
TACACTG94400.011.8274325
GTATTAT117400.011.7952461
GTATTAC53500.011.5187911
TAGACTG79900.011.433145
GTGTAAG74200.011.2366191
CCTACAC53300.011.1539423
CTAGGAC51750.011.0677283
GTCCTAC35400.011.0594171
Produced by FastQC (version 0.11.5)