FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGGAGCAGAAAGCAGAGAGAAGTGGCTAAATCAAGCTGCCCTTGCCT11.8181818181818181No Hit
TGTTGGAAGAATCCATGGTTTGAGGGCTTCTAGAAGGAACTGGAATGTTT11.8181818181818181No Hit
CTCTGATGATAGTTTCTTTTGCTGTGCAGAAGCTCACTAGTTTAGTTAGA11.8181818181818181No Hit
CGCTGGTCATGGGAGCTTTTGCCGTGATTCCAATATACTCGGTTCGCTTA11.8181818181818181No Hit
GAGTGGAATAGAATGGAATGCAATGGAATGGAAGGCAAATGAATGGAATG11.8181818181818181No Hit
TCCCAGGACTCTGGGAGGTCAAGCTGAGCGAATCACTTGAGGTCAGGAGT11.8181818181818181No Hit
CACACACACCCCTACAGGCCAATATCATTAACGAACCTAGATGCAAATAC11.8181818181818181No Hit
GTACTGATCTCGAGGCCACTGCTACCACTTTCACCATCTTCTGTGCCTCA11.8181818181818181No Hit
GCCCCGGCCCGGGCCCGCCCGCCGGGGCCCCCGGGCCCGCCGCCCGCGCA11.8181818181818181No Hit
AATATTTACTGTGCAACTTTCCTAAGCAAGAGACTATGCAAAGACTTAGG11.8181818181818181No Hit
GTGCTGTGAGACAAGGACCAAAAGAACCATATGTAGATTTTATAGCCAGG11.8181818181818181No Hit
CTCTTACTACGGTGTTGAACTTTATGGCGATGGACTATCATCAGTTACAC11.8181818181818181No Hit
ACATAATATTAATTTACTTTAAATTCACAGGTGGCTCATGCCTGTAGTCC11.8181818181818181No Hit
ATATATTTTTAGTCATGAGTAAATTTTATTTTATTCTAACTCTTAAAAAT11.8181818181818181No Hit
GCGGCGCCCTGGAAAGCCCTCTTTTGAAAGTACCTTCTGAGCAAAGCCAC11.8181818181818181No Hit
TATCGAATGGAATCAAAGAGAATCATTGAATGGACTCGAATGGAATCATC11.8181818181818181No Hit
ACCCTGTGGACCGGCCATTCGGCGGCCATCCTGGCACCGCTGTCCTCGCA11.8181818181818181No Hit
GTACAGTTCACCCTAAGAAAAATATTGGACTTACATAACTAAAGCCACAT11.8181818181818181No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.8181818181818181No Hit
CATCGGCTGCTCCAGGCTAAGCTGGTCAGCACTCTGAGGCTGGAGGTCCC11.8181818181818181No Hit
ACTCTGGAGCATGCTGAGATTAAGGTGGTTGCCAAGGGAACAGAAAACAG11.8181818181818181No Hit
ATCTTATCTTCATCTAGCCTGTGTTCTAAACATAGCCAAGCTGAGAGGCT11.8181818181818181No Hit
AGGTTGTCTTCCAGGGTTTTTATATTTTTGGGTTTTACATTTAGGCCTTT11.8181818181818181No Hit
CTGCATCCTCTGCCTTACCTGCGATGATGTTCTGCGAACCCAAGTCTACA11.8181818181818181No Hit
CTTTATACTATGTCTTTTTTTTTCTAATGGAACCTATTCCACTAGATTAG11.8181818181818181No Hit
CTGGATCCTCGTAATCAGTGTTATACAATACGATTAATGGCACATACTCG11.8181818181818181No Hit
GCCTTTACATCAGGAACATCAGTGGGCCTCAAGTGCACATCTCTTGTCCT11.8181818181818181No Hit
GTTATATGCTGTATACTTGTCTGCCTTTTAAGACAGAATACACTGAATTT11.8181818181818181No Hit
GCCCCGGCGGTCGCCACGGCGCCGGGCGCGGGGGGAGGCTCCGGGGGCGG11.8181818181818181No Hit
CGAGGGTACCCCCTCGGCTGCGCTGAAGTCCCAGCCCATGTTCTGGTACG11.8181818181818181No Hit
GTGGGTGGTGGGCTAGGGGACCGATAGCATTAGGAGAAATACCTAATGTA11.8181818181818181No Hit
GGGTCATTCCACTTCTTCTTAAATACCTGCTGTGATGGAGATCTCACCCC11.8181818181818181No Hit
GGTCTCGAACTCCTGGGCTCAAGCGATCCACTCACCTTGGCCTCTGAAAG11.8181818181818181No Hit
ATCTAATGGAGATGGTAAGTAGTCAACTACTGCATCTAACATTAATTGTA11.8181818181818181No Hit
GGCTGAGGCAGGAGAATGGTGTGAACCCGGCAGGTGGAGCTTGCAGTGAG11.8181818181818181No Hit
TCTGATTTTCACTCTAAGGAAAGCCAGCTCCTTTGTAAAAGCACTTAATC11.8181818181818181No Hit
ACTCGGGAGGCTGAGGCAGGAGAATCTCTTGACCCCGGGAGGCAAAAGTC11.8181818181818181No Hit
ATTACCAACTATTTTGTTTTTTTGTTTTATTCATTCACTCATCTGGCTTA11.8181818181818181No Hit
AGGTATTGATGGAACATATCTCAAAATATTAAGAGCTATTTATGACAAAT11.8181818181818181No Hit
GTATAGTGGTGCACACCTGTGCACCCAGGCACTCAGGAGGGTGAGGCAGA11.8181818181818181No Hit
CTATTATCATCTTCATTCTACAGATGAGGAAACTGAGGCACAGAGAGGTC11.8181818181818181No Hit
CTTCTAGCTTACCCATAACGTCACTCACAACCTTAGGCATGGGTACACAC11.8181818181818181No Hit
GGACAGGAATGTTGACTGTCTTTTTCACACTGTATCCCCAGCACTTTGAA11.8181818181818181No Hit
CAACCTACCGCTGGCCACTCCTCGCAAGAGGGAGAGTGTAAGCAAGTGAG11.8181818181818181No Hit
GTATTTGTCAGATCCTGCTCAATTATAAGATGATCTTTATCTGTCCGCCT11.8181818181818181No Hit
GAGAGGGAGAGAATGATAGAGGAGTCAAATGGTAACACTTGCAGAATCTA11.8181818181818181No Hit
ATTACCTTATTTTCTTGCTACCCTTTAATAGCCAAACTTCTTGAGAGACT11.8181818181818181No Hit
CATCTGCTAAATGGTCTCATAAGTGTCAGCACCTTCAATGTCACTTGCTT11.8181818181818181No Hit
ATATATTTCTTTGGCAGGAAAGTATATAGAAGAACAGTATATAGAAGAAA11.8181818181818181No Hit
GGCCCGCCCGGGGGGCGGCGGCCGGGGGCAGCTCATGGGCGGGCCGGCGG11.8181818181818181No Hit
GTTTTGATTTGCATAAGAAGTAATTCTCCTACTCCTCTGATACGCCTATT11.8181818181818181No Hit
CTTCCCCCACAGCCAGGAGGTCAGCTCTGCCTGCTGTTTGTAAGGTCTGG11.8181818181818181No Hit
TCCAAGTCACTCAGCACCAAAGTATGTTAGTGGTAAAAAGTACCTGAGTC11.8181818181818181No Hit
CAGCACGAGCAGTCGGCTAGCTGGGTGCATTTCCCGATTTTTGGTTGGTT11.8181818181818181No Hit
CAGACAAATAAAGATACATATTCAACCTTATATTTTCATATTTAGACGGT11.8181818181818181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACTC50.0145.08
ATCCAGA50.0145.0145
TGATTTT50.0145.03
TTTTCAC50.0145.06
TTTCACT50.0145.07
CTGATTT50.0145.02
GATTTTC50.0145.04
TCACTCT50.0145.09
ATTTTCA50.0145.05
TCTGATT100.072.51
Produced by FastQC (version 0.11.5)