FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12209239
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG1700.003094353221.3240094
TATACTG223050.015.4072075
GTATTAG94900.014.9739941
CTATACT105200.013.6457454
TACACTG123300.013.23022655
ATACTGT260400.013.0859336
GTATATA429200.012.7367741
CCTACAC56400.012.7263523
TCTATAC101600.012.6306983
GTATAGG50100.012.4453911
GTATAGA97600.012.3312211
TATACCG11851.8189894E-1212.2365635
GTATAAG80700.012.2183681
GTCCTAC40050.012.1288631
TATACAG135200.012.1193575
CTAATAC93700.011.9933343
ATATACT282600.011.8270554
CCTATAC52200.011.8058213
GTCCTAA67700.011.7801781
GTATTAT199900.011.5697961
Produced by FastQC (version 0.11.5)