FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25901029
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG174350.016.550645
GTATTAG174850.015.5909171
GTATATA285000.015.56883051
GTGTATA238250.014.5155981
GTATTAT216050.014.3292361
GTATAGA150050.014.2539591
TCTATAC142300.014.1132983
GTATAGG100750.013.9606661
TATACAG195200.013.85423855
GTATAAG149650.013.7106861
TATATAC290600.013.3728143
GTGTAGG133400.012.88077451
TATTATA277900.012.8621052
GTATAAT195300.012.7704341
CTATACT146650.012.6564414
TTATACT210400.012.6121464
GTATTAA205550.012.5921581
GTGTAGA181800.012.4026921
TAATACT199950.012.2922654
ATAATAC210200.012.1067633
Produced by FastQC (version 0.11.5)