FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20416730
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA277900.014.4793721
TATACTG158500.014.2258965
GTATAGA134050.014.0621541
GTGTATA194950.013.2395151
GTATTAG143400.013.094711
TATACCG22850.013.0090675
GTATTAT211600.012.6774351
GTATAAG130900.012.2404661
TATATAC282500.012.1392313
TATACAG170100.011.8065765
GTATTAA190150.011.7435581
CCTATAC84150.011.5451393
GTATATG180450.011.4507331
GTATAGG82700.011.3968061
GTCCTAG94600.011.1894051
TAATACT196400.011.1484364
TATTATA333700.011.1240332
GTATAAT200850.011.0457421
ATAATAC207200.011.0222263
TACACTG165550.010.861035
Produced by FastQC (version 0.11.5)