FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_195.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_195.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGGACCTACCGGGCTGCTTCCCAACTAATGACTGAGCACAGTGAGG21.9607843137254901No Hit
AAATAAGATAGCATGTGAAAGCAGTTCTAGATTCCAGACATAAGAGTAAG21.9607843137254901No Hit
GTCACATGCCAAGGCAGAAATTACTCGTAGGCTCTCCTGACGGATGTAAG21.9607843137254901No Hit
CTCTGAGCCTTCTTATCAAAACTCACTTCAAGATCTTTGCTTTACTCTTA21.9607843137254901No Hit
ATATTCATTGATGTATGAATAAAGAGATTTGAAGTGGCCCTGGGACGACT21.9607843137254901No Hit
CACTTTATGGTCTTAAATACACAGTAGGCATTAGATATTTCTTTTGGGGT21.9607843137254901No Hit
CAGTTAACTGTGTAGGTTAAACATCGAATGTCAACGGCCAGGCACGGTGG10.9803921568627451No Hit
AGGTGAGGGACCGAATTTTAAAAACAATTCACCTTATATACCCAACCACA10.9803921568627451No Hit
CTTTCCTTCTTTCTCTTGTGGGCATTTAGTGCTATAAATTCCCTCTACAC10.9803921568627451No Hit
GTTATGCAGTGGTTAAGTTCATGACACATCTTGTAGAAGTTTTAAAATAT10.9803921568627451No Hit
CGCTGGACCTGACCGGAAGAAAGGGACGAGTTGAGGAAGCCTGTCCCTCA10.9803921568627451No Hit
GATTACACGCGTGAGCCACTGCACCCAACTACTCTCTGATCTTTTACAGA10.9803921568627451No Hit
ATTAAACTTCCTCAGCCTCCCTGGGCAGCCAGAGGGCAGAAAACTCACTG10.9803921568627451No Hit
GGTGTCAGTACTACTGAACTGTGCAATACTATGAAAGCCACTCATGTGCA10.9803921568627451No Hit
AGACAACCAGTCGAGAAGAAGTCCAGGGTCGCCAACTACGTGATACGGTC10.9803921568627451No Hit
CCCATAGGATGTGGTGGAACAGGGCACATAATGGACAGAAGTGGGAGGGA10.9803921568627451No Hit
GATTCCATTCCATTCCATTCCATTCCTTTCCGTTCTATTCCATTCCATTC10.9803921568627451No Hit
GACAAGTCAGAAGTCATAATTGTATGTTTAAGAACAAAAATTCTTTAGAT10.9803921568627451No Hit
CAATACTTAAACAGTTAAAAACAGAAACTTCAAAGATTCCTTGTACACTA10.9803921568627451No Hit
CATGTTTTAAATTTGAGTATATAAGTTTTTAAACAAATAAACAATTAACA10.9803921568627451No Hit
CTATTGCAATCATGTTCCTTTGGAAAACAAACGTTAGTAATAATGCTCAC10.9803921568627451No Hit
GAATACAAGTTCCTTATTAAATACCTGATGTGCAAGTATTTTCTCCGTCT10.9803921568627451No Hit
TATGAAAACAATAGAGGGTCATAAACACAGAGATTATAAAAATTAAATAA10.9803921568627451No Hit
GTGTGGTGGTGGGCACCTGTAATCCCAGCCACTCAGGAGGCTGAGGTAGG10.9803921568627451No Hit
GTCTTTCCTATTAGTTTCAAATTCTGTTGTCAAAGCTGTGATTAATTTAT10.9803921568627451No Hit
CTAAACAAAGCTTTTTGAACAAATTCGAGATTCAATTTCATGTTACCAGC10.9803921568627451No Hit
GTGTCGATTTTCCACCAGATGAACATGTATAAAAATCGCCTTATAAATGA10.9803921568627451No Hit
GGGCACGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCA10.9803921568627451No Hit
GGGTTGCGTGCCTTTATGCACAACTCTGTTTAGAATTGCATCTCTGATTT10.9803921568627451No Hit
CCCCATTTACCGTGATGTAATTATTATACATCGTATGCCCATAGAAAAAT10.9803921568627451No Hit
GAAGAAATCCCGTTTCCAACGAAGGCCTCAATGCGGTCCATATATCCACT10.9803921568627451No Hit
GTGTTGTTCCTCTCCCTGTGTCCAAGTGTTCTCATTGTTCAACTCCCACT10.9803921568627451No Hit
CATCTGTAAAAGGTGGGGGGTAAATAATGCCTATCTTGCTAGGTTGTTGT10.9803921568627451No Hit
GTCAAAATGGGGTTACAGGTGGACATTCTACTTACTCTCTCATGCATTTA10.9803921568627451No Hit
GTGTGCGTTGAACCCAGAGAGATGAACCTTTCCTTTGATAGAGCAGTTTT10.9803921568627451No Hit
CAAATGCAATCTAATCAAATGCAATTAACCCGTAATGTATACAATCGCAT10.9803921568627451No Hit
TCCCTGCACCTAGTGTTTTGAGATCTATACAATTAATTAGCTCAGAATTA10.9803921568627451No Hit
CGTGAAGGTGACTCACAATTTGTATATGAATTCATAAAACCTGACAAAAA10.9803921568627451No Hit
GGGATGGGTGAATGTATTGATGGTAGGGTGAATGAAGGGATGGGTGGATA10.9803921568627451No Hit
TTGCAAAATTGAACTTGGATCCATCCAAAGGTGGTTTATAGAGCACAGAT10.9803921568627451No Hit
GAGCACTCAAGCATTTAAATAAAGTATAAATAAATCATCAATAATAAGCA10.9803921568627451No Hit
ATCTTATGCCTTACACAAAAATCAACTCAAAATAGATTAAAGAAGTAAGT10.9803921568627451No Hit
GTGGTTTCTGACTCGTTTTATGACGCGTAAATTCTTGTCCGGCATTCGAG10.9803921568627451No Hit
GGGTTAATTTCAGTTGTATGTGAATTGTGTTTTCACCAGAGATGTGTTGT10.9803921568627451No Hit
CTCCAATGGAATCATCACCAACTATAATCAAAAGAAATCATCAAGTGGAT10.9803921568627451No Hit
CTATGTTGGCCAGGCTGCTCTCGAACTCCTGGCCTCAGGTGATCCACCCG10.9803921568627451No Hit
GCTCTGCGGCATGCAGAAGCAGGCCATATTCCACATGCGGGTGTCCCTGC10.9803921568627451No Hit
GTGGCATTCCGGCTACATACCAAGATAACATGACCTGTTTAAGATTATAT10.9803921568627451No Hit
GTGAATAGAGTAACCAAAAAAAAAAAAAATCTGATTGAAGCTAAAAATGA10.9803921568627451No Hit
CCACTGTAACTTATAAATAATGCCACCTGCTGAGTGTTCAACAGAAAACC10.9803921568627451No Hit
GTCTAGGGTCATAGGAAGAAATCCCGTTTCCAACGAAGGCCTCAAAGAGG10.9803921568627451No Hit
GTGCTGGGATTATAGGTGTGACCCACTGTGGCCGGCCATTATTTCCTCTT10.9803921568627451No Hit
GGCGAAAGCTACATACTCCTCCAGGCCCTGCACATTCCTTTCTAAGGCAG10.9803921568627451No Hit
TCCTAGGAGCTCTGCTTCTCTGGTTGAGCCCTGACTAATATAAGTGCTTA10.9803921568627451No Hit
ATGTATAATTATACATGTGTAATTATATTATACATGTAAAAATACATGTA10.9803921568627451No Hit
GCACCCTACCCATCCCACGCCAGCCAGAATGGTGTTGGACAAAACCAGAT10.9803921568627451No Hit
ACATGAAATTCACTTCTGTTTCACATACACCTAAAAAAATCTATCACAGC10.9803921568627451No Hit
GCCTGTAAAACAACAAACGACTTTAATTATACTTATTCAGTATATAATTA10.9803921568627451No Hit
GGGTGGAGTGGAGTGGCAGGATCTCTGCTCTCACCTACCTCTGCCTCGGG10.9803921568627451No Hit
GGCGAAAGCTCCCTCCTGCTCCAGGCCCTGCAGATTGCTTTCTAAGGCAG10.9803921568627451No Hit
CAGTGTGGTACTGGCAGAAGGACAGACACATAGACCAATGAAACTGAATA10.9803921568627451No Hit
GAAAACATGTTGAGAAATTCGTGGTCACACACTTATCAACTTTCGTAATA10.9803921568627451No Hit
GTTCTCCTACTGCCTGTCACACAGCTAGACTGGTTATCCCGTGGAGGAGT10.9803921568627451No Hit
GAACAGGAAGCCACTGTCAAGGAGTCTAAGAAAGGGAATCTGCAGACTTC10.9803921568627451No Hit
TGTGGAGCCAGCTCTTTATCAGCCTACATTTCAACTCTTACCATCTAAAA10.9803921568627451No Hit
AGCCTGGGTAACAGAGGGAGATTTCGTCTCAAAAAAAAAGAACTGTGGGA10.9803921568627451No Hit
ATAATGAGGGAAGCACACCATTCTTATAAGCAGGAGTGAAGTCATATCCA10.9803921568627451No Hit
ATTTTATCAGAGGCTTTTTCTGCATCTATTGAGATAATCATGTGGTTTTG10.9803921568627451No Hit
CAGGGACAGCCTACATGCAGGTCACGCCCAAGCCACACTTTGCGGATTCA10.9803921568627451No Hit
GTCTTGCTCTGTCATCCAGGCTGGAGTGCAGTGGCACGTCTCGGCTCACT10.9803921568627451No Hit
ATATTTTTAGTAGAGACGGGGTTTCACTGTGTTAGCCAGGATGGTCTCGA10.9803921568627451No Hit
ATTATGTCCTCAGTAAATAAACTAGAAATAGAAGTGATCCCATTTGGGGA10.9803921568627451No Hit
GCTCTCAATCTCTACAAAGAAAACTTAAAAAGATCCCTAGAAAGGAATAA10.9803921568627451No Hit
CTCCTCTTTAGAACACCGTCAAGTCTTCCAAGATCTTTGCAGGGCTGGAA10.9803921568627451No Hit
AGCCTGGGCAACAGAGGGAGATTTCGTCTCAAAAAAAAAGAACTGTGGGA10.9803921568627451No Hit
ACCATGTAGCTGGGATTACAGGCGTGCACCATCACACCTGCCTAATTTTT10.9803921568627451No Hit
GCCCTGGGAGGCTGGACCGAGGGAGGCTGGACTTCCCACTCCACCCTACA10.9803921568627451No Hit
TCTTTCTGGCTTTACAGTGTAAACCAGTACAGCCTTGATAGCTCCTTGAG10.9803921568627451No Hit
ATGAAAAATTTCTCTATTTTGTACAACAACCTCCTAGGCTCAATACATTT10.9803921568627451No Hit
CTATTCGACCAAGCCAATTATTATATCGATTTGCTGCATTACGAGCGTGG10.9803921568627451No Hit
CTCTCAACACTGGCTTCTCTGGCCTAGAAAGACCTAGAGGTGGCTGCGGC10.9803921568627451No Hit
CAAATTTAATGTACAGAAATTAGTAGCTCTGCTTTAACGTAACAGTGACG10.9803921568627451No Hit
CAGGCAGCATGAAAAAGCGAACTGATTAGAGTCTAATGGGGGTATTACCA10.9803921568627451No Hit
GTGGAATCCCTGAAGGTCCAAATCTTACGCTTACTTTCGTATATTTCTTA10.9803921568627451No Hit
GTTTTTTTCTTTTAGGATTCCTCAAATACATGACAACATCTAATTTCACC10.9803921568627451No Hit
GTATCAGCCGCCACCAGCACTGAATGATGCAGGCACTGAGGGCTGCGTGC10.9803921568627451No Hit
CATCTAATAGTAAAACAGAAGACACTTTTTTATGTATAAAATATGACTTT10.9803921568627451No Hit
ATATCCAGTCTGCTAGTGGGAAGAAGAAAAGAGGAAGCTTAAGGCAAAAG10.9803921568627451No Hit
GTGCTGCAGGGACTCCTGCATTTCCTTACTAAGCCTTGAGCTTTTAAATG10.9803921568627451No Hit
CCGTGTCGTACAGTCAACGATGATTCCCTTATCTAAAATTCACTCAGTCC10.9803921568627451No Hit
ATCCAGCTTTATATTTAGAAAGGGAAGAAATGAAAACAGCCAATATTACA10.9803921568627451No Hit
GCCATACACGTGCTTGAATCACCCCTGCCCCCAAATCCAGGCAGTTCAGC10.9803921568627451No Hit
ATACAGGTGATTCACCACCTAAAAAGAATTAAAACAAAAACCAAATGATA10.9803921568627451No Hit
AGTACCCCTACTCAAAGCCCTCAAGCCCGAGGCATCTACCCTGCGCCGTG10.9803921568627451No Hit
GGTGTCGTGTGGTGCTGGACTTGCTTCCGCTGGAAGGAGAATTTTAGGGA10.9803921568627451No Hit
GGCGTTAGCACTTAAACGATTATACATGTAAATATAACTTCTCACATCGT10.9803921568627451No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers