FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22031117
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA345000.016.0132831
GTATTAG161250.014.5676591
GTATAGA144000.014.50021651
GTATTAT223650.014.1339021
GTATTAA205500.013.5829281
ATAATAC220300.013.2299153
GTATAGG86200.012.9526311
CCTATAC87000.012.8335543
TATACTG177250.012.5164645
TAATACT213350.012.5055174
TCTATAC137850.012.4122553
GTGTATA256000.012.3195211
GTATAAG147800.012.312431
TATATAC348450.012.2968113
TATACAG190200.012.2360425
CTAATAC156450.011.9097613
GTATAAT217350.011.9083911
TATTATA344050.011.7797412
ATTATAC200300.011.6190213
TATAATA357050.011.3914582
Produced by FastQC (version 0.11.5)