FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23170413
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG94400.014.9765571
GTATATA353850.014.2811431
GTATAAG144250.014.0731571
GTATAGA147650.013.3562581
GTGTAGG112950.013.3513891
TATACTG184700.013.1110185
GTATTAG159800.012.9305631
GTGTATA248800.012.7927381
TAATACT218200.012.5601244
GTATAGC77450.011.9822381
CCTATAC88350.011.9812273
CTAGTAC75700.011.9720663
CTAATAC157950.011.88872053
TCTATAC141050.011.7711093
ACTGATC192550.011.7477548
TACACTG207250.011.68446355
TACACCG23600.011.6742355
CTTACAC156600.011.4819313
TATACAG208550.011.4725685
GTGTAGA163800.011.3311881
Produced by FastQC (version 0.11.5)