FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16684324
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG120450.016.0714631
GTATATA318650.014.2660761
GTATAGA112750.014.0824591
TATACAG159600.013.582885
GTATAGG64950.013.3953181
TATACTG167800.013.3079845
GTCCTAG80900.013.0844441
ATGCCGG34200.012.931207145
TCTATAC110150.012.6377663
CCTATAC65750.012.3502523
GTATTAA167200.012.2282351
GTATAAG105750.012.0665531
GTATTAT194600.011.996761
CCGTGCG9109.140422E-911.95058259
CTATACT120650.011.89847854
ATAATAC194100.011.6168513
GTCCTAC54650.011.5419731
GTGTATA201650.011.5054261
GTCTTAG98150.011.4496541
TAATACT178450.011.4167724
Produced by FastQC (version 0.11.5)