FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15320978
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA206800.017.1789041
GTATTAT149250.015.3019281
GTATAAG100950.014.1484811
GTGTATA150850.013.553571
GTATTAG108700.013.3398321
GTATAAT135700.013.3036071
GTATAGA89100.013.1008081
TATACTG116850.012.6576415
GTATTAA138650.012.4976381
GTATAAA182600.012.1895461
TATACAG123650.012.0788175
ATAATAC143000.011.91470053
GTATAGG60900.011.9050881
TTATACT144650.011.728674
GTATATG151550.011.5295141
TCTATAC86200.011.5229753
TATAATA213300.011.4208852
TAATACT138600.011.4036694
ATTATAC129700.011.4035883
TATTATA211950.011.3910072
Produced by FastQC (version 0.11.5)