FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAGAGTGTGCTTTTCGCCGGTGATAGACGTTGTAATTTTAGCTTTA13.4482758620689653No Hit
GGGCGGGTGCTGTGTGGGGGGGCCCCGAGCTGGGGCGTCGTCGGGTAGAT13.4482758620689653No Hit
GCTTTATACAGCAGCCAGAGCACCATGCCCCTGCTTACACTCTCCAACGG13.4482758620689653No Hit
CTGTACAGAGTGACCGGGCATGGTCGAGCCCCAAAACAAGGCTAAAACAA13.4482758620689653No Hit
GTTAAATGGGGTACACAACTAGGTATTGCAGAGATGCTACGTTCTGGTAC13.4482758620689653No Hit
TGTTTTAACTGTGCTGATGGAGGCAGCTTGGCATTCCTGGGCTCCTTGAA13.4482758620689653No Hit
ATCTTGGCTCACTGCAACCTCCACCCCCCGGGTTCAAGACATTCTCCTGC13.4482758620689653No Hit
TCCTAGTACTGAACTTGGATTATGCTTGCCTGCCAGTATAAAGTACTGAA13.4482758620689653No Hit
AAATTACATATACTTTCAGAACCCTATGATATGTTGATTAATATTATAAC13.4482758620689653No Hit
GAATATTACATGGAAATAACTGAGACCTGATAGTACGCTGCTAAGGTTTG13.4482758620689653No Hit
CTCGGATATCGACCAGCTTTTAAGTCGACATACCACGTCCATTATCGACC13.4482758620689653No Hit
GCAGAGTCTGAGGAACTGGCCACCTCAGTCAAGGACTCAGGAGGCTGCTT13.4482758620689653No Hit
CCTCTTAAGAGCCTTTCTAAGAACATGATAGGCTTACTTCTGCTCCATAA13.4482758620689653No Hit
ACTTAGGAATGTGCCTAGCACATACAGAGTGCTTGATTTTTGGTTCATTG13.4482758620689653No Hit
ACATAAATGTATTCATTTGAGAAGTGTCTGTTCATATCCTTCACCCACTT13.4482758620689653No Hit
ACACATTCTTTTGCAAACTTTCAGAGGCTAGTTTCAGTTAAAACAACTTG13.4482758620689653No Hit
CTCTTTAACATTGCTGAGCCTCTACAAGTGGTGTTCGGTGCACTCACCTA13.4482758620689653No Hit
AATTTTTATTATTCCTTATTATCGACAAAGTAATTAATTTATAAAATCAC13.4482758620689653No Hit
CAAGACATCCACAGTAGTTCTCCCTCCTCTCTTCACAAAGTCAGTCTTCA13.4482758620689653No Hit
GATATACTTAGAGTAAAAGCTGTCATTAAAAATGGACATTTCAAAGCCTA13.4482758620689653No Hit
AAACAAATATTAAATCAAAAAAAATGGTATGTAAAACCTCATACAATAGA13.4482758620689653No Hit
CAAAACGTGTACATATTCTCTCTTTTTTTTTTTTATTTTTTATGTCGCCG13.4482758620689653No Hit
GTTCTTGCATTGCTGTAAAGGAATACCTGAGACTGGGAATTTATAAAGAA13.4482758620689653No Hit
TGAAGAAACTGCAACGAAAACCAAAAAGGCAAAAGAAACTCCAATCAAGA13.4482758620689653No Hit
CAATAAAGCAAGCAGTTCAGTGCAAGCCCACGGCTCTACAGCAAGAAACT13.4482758620689653No Hit
GCATACGCACGGAACCTGGTGCTCTTTAACAACCTCCACCTCTAACTCAT13.4482758620689653No Hit
CCCTTCAGAGTTCAACATTAAATAAACACGCAGCCATTCCACAAAAGAAA13.4482758620689653No Hit
CAAATAATATTTAAGTTAAGTTTATTCAAACATCTTTTTTATTAACTTTT13.4482758620689653No Hit
TATATAAACAGCACATTCTAACTTTTTTTTTAAGTTTAGCATCTGGACAC13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)