FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15164813
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG103650.016.5077251
TATACTG130950.016.4989645
GTATATA229600.014.2097061
GTATAGA105750.014.0545841
TATATAC232650.013.2443863
TCTATAC106750.013.2438013
CTATACT102050.013.1433074
GTATAAT157450.012.75503351
GTATAAG107450.012.3477881
TTATACT164150.012.234464
ATATACT186150.012.2296094
GTATTAC94400.012.2115171
GTGTATA149650.012.1117751
GTATTAT180700.012.0768271
GTATTAA152500.012.075591
TACACTG114500.011.9674675
TATACAG138250.011.85193355
GTACTAG45800.011.555851
ATAATAC177150.011.5003513
GTCTTAG85250.011.4811111
Produced by FastQC (version 0.11.5)