FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4967663
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC4058.046349E-616.1110343
TATACGC4251.2707358E-515.3530215
TAATACT51450.014.9367594
GTATATA94100.014.40747451
GTCCTAC15400.014.1233071
CCTACCG5205.5016008E-613.942389
CCTATAC19400.013.8272533
CTAATAC36750.013.8094573
GTATAAT51250.013.7218851
CTATACT34300.013.5276314
TGTACCG3800.001454141213.3553325
CGTGTAC4352.6548328E-413.3332693
TATACTG46750.013.1818865
TCTATAC32450.013.1817543
CGTCTTG16550.013.141931145
GTATTAA48000.012.9895191
GTATTAT56450.012.5862981
GTACGGA7601.369026E-712.4012561
TACTACG4100.002574951412.3779892
GTCCTAG23700.012.2362271
Produced by FastQC (version 0.11.5)