FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948454
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA11000.019.7726761
ATAAGAC6653.6379788E-1217.4435653
CTATACT5201.792614E-816.7307264
GTCCTAT4058.032432E-616.111071
CTAGGAC2700.002331309516.1110693
CCCTAAC3203.9524745E-415.8593341
CTAGCAC4151.01241585E-515.7228523
ATACCGG2800.002971313715.5356736
GTCTACG3355.598675E-415.1492151
TCCTACA7202.746674E-1015.1041282
CCTATAC3859.3970644E-515.0648973
TAAGACG2900.003753341314.9999624
GTCTTAC4852.6660964E-614.9484151
TCCTATA5405.005786E-714.768482
GGTCTAA2950.0042051514.7457251
ATTATAC8401.2732926E-1114.6725833
ATACACC6451.5899786E-814.6123664
ATTATGC7957.8216544E-1114.5911583
GTCTCAC6001.0401527E-714.4999631
GTACTGG7002.9849616E-914.4999631
Produced by FastQC (version 0.11.5)