FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18616050
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG131300.016.0130831
GTATAGG80650.015.7316891
GTATAGA126000.015.70846751
GTATATA339150.014.85712051
TATACTG171950.014.6730735
GTATAAG126250.013.6674431
GTATTAT201900.013.4300311
TATACAG176450.013.35382655
GTATTAA182650.013.2974021
TCTATAC120100.012.9789123
GTGTATA224650.012.9736251
CTATACT124450.012.7000574
CCTATAC77450.012.6373263
GTACTAC56450.012.5864671
TACACTG175900.012.4888035
GTCCTAG95400.012.235431
GTGTAGG93250.012.0510411
CTTACAC131700.012.0008943
TAATACT189450.011.8251854
ATTATAC182950.011.5319693
Produced by FastQC (version 0.11.5)