FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11032885
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA165500.014.500451
GTATAAG88900.014.1905421
GTATTAT148800.013.3992741
GTATTAG88950.012.8784211
GTACTAG30950.012.6498281
TATACAG106850.012.6208885
GTATAGG59850.012.5985531
GTATAGA95950.012.5433811
TATACTG97800.012.5285085
TAATACT129200.012.1772364
GTCCTAT58750.012.0939921
TCTATAC90250.011.8895653
GTGTATA108600.011.7499051
CTATACT85850.011.654414
GTATACG31150.011.6376011
TATACCG32600.011.5647755
GTATAGT73750.011.5020511
GTCTAGA52250.011.3783441
GTATAAT125100.011.2433551
GTATTAA122950.011.2040881
Produced by FastQC (version 0.11.5)