FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14945072
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT64750.014.6681621
ATGCCGG25950.014.527854145
GTATAGA105900.013.9662051
TATACTG167200.013.9626595
GTATATA318050.013.9508341
CCTATAC59750.013.5902883
CTATACT110450.013.1940734
CGTCTTG27250.013.036621145
GTACCGT10653.6379788E-1212.9345866
GTATTAG108400.012.8415111
GTCCTAA78100.012.6250191
TCTATAC106350.012.5437933
TATACCG11801.8189894E-1212.2884345
GTATTAT188400.012.2759181
GGATCGT23650.012.262298
GTCCTAG70550.012.1263251
TATACAG146250.012.0960215
GCTAGAC87400.011.945373
GTCCTAC46850.011.9158581
TAATACT159000.011.5364444
Produced by FastQC (version 0.11.5)