FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAACTTAAAACAAAAAATTATTCAAAGTAACGTCAAAATCATAAA11.9607843137254901No Hit
CCCCTAATCCCAGGTTTTCCCCTGGAAGTTTGATAGGCTTCTCCTCTAAG11.9607843137254901No Hit
GTAACTGTTCTTTGACTTGGTTTTCTTTTTCATATGTAAGATGAGAATAA11.9607843137254901No Hit
GCTCTGAGTTATCCAGACACCAGACAAGTTTCCTGGGCTTTAAACTCCCA11.9607843137254901No Hit
GCCTTAATTTGTGCAGATACTGATGGAAGACAGGAGGACCCACCTTCATC11.9607843137254901No Hit
ACCTGGGTGTGGTGGTGCATGCTTGTAATCCCAGCTACTCGGGAGGTTGA11.9607843137254901No Hit
GGTGGGGCCGCGTGCCCGGCGGCATAAGTGAGATTATATCATAGTCGAAT11.9607843137254901No Hit
GAATCAAATGGAATGGACCAGAATGCAGTGGACTGGAATAAAACGGACTC11.9607843137254901No Hit
GAGAAATATTACGCGACTTGCTCAGACTTGTATAGACAGCCAGTGGCAGC11.9607843137254901No Hit
GTTCGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTGCACTC11.9607843137254901No Hit
TCATTGAATGGAATAGAATGGAGTCGTCATCGAATGGAATCGTATGGAAT11.9607843137254901No Hit
CTCCTAACCTTTCCTTTCTACCCCCTCTCTTTCCCTTCTCTCTTCCCCTT11.9607843137254901No Hit
ATGTAGGACACACCTGTGCATACATAACTCTTTCCTGTTTAACATGCTGT11.9607843137254901No Hit
AAATGCACACAGGTCTTACACACTAAACCCAGTAAAAACTGGGAAAAGAT11.9607843137254901No Hit
CTTTTTGTAAGTACCAAAGGACAATACACATAGAAGGATCACATTAACAG11.9607843137254901No Hit
GATAAAAACTAGTATTTCAGCCTAACATTGAGTGTACTTATAGCTCACCA11.9607843137254901No Hit
CATTTGGAGCTCTTGGGTTATATTTACTGCATTGCCAGAAGCCAGCAAAG11.9607843137254901No Hit
ACAGACCACTGTGATTGGCAACCTCACTAGAATCATAATTAGAGAGACGG11.9607843137254901No Hit
TAGAAATTGTTAGTAGAGAAGATGGTTCTAGGCAGAGGGAAACGCACGTG11.9607843137254901No Hit
ATATTAGTGCTAGTGATAGTATTAATACAGTAAATTAAACAGTTTGGAGC11.9607843137254901No Hit
CATGTATTCTCATTGTTCAATTCCCACCTATGAGTTAGAACATGCGGTGT11.9607843137254901No Hit
CTACTAAAAATACAAAAATTAGCTGGGACTGGTGGCACGTGCCTGTAATC11.9607843137254901No Hit
CTTAACGTGTGTTCCCATAGCTACACTCACAAAATGCCACAGAGCCTTCC11.9607843137254901No Hit
CCATACAAAAATCAAATCAAAATGAATTAAAGACCTAAATGTAAGACCTG11.9607843137254901No Hit
GAATACCACTGACACCTCTGCAAGTTAAGCCTGTGGAAATAGTGTTCCCA11.9607843137254901No Hit
GAATGGAATGGATTCGAATGGAATGGACTCCAATGGAAAGGATTGGAATG11.9607843137254901No Hit
GTCTTCCTAAGGTATATTACTCACCCCACTCTTTTGTTGAGGTAGTTACT11.9607843137254901No Hit
GTATTGGGCCCACCTGACTTTCCTCTTTGCTGTGGCATCCCCATCAGCAT11.9607843137254901No Hit
ACCTCAGCCAGCCTCCAAAGTAGCTAGCACCACCATGTCTGGCTAATTTA11.9607843137254901No Hit
GGTCGGGAGATTTTGAGTGTACAGCAAAAACCTCTGAACTTCCATTTGGA11.9607843137254901No Hit
CTATTTGAAGACAATTTAAAAACAGTTGTAGAAAGTACTCCTAAAATAGC11.9607843137254901No Hit
ATTTATTTCTGGGTGGTCTATTCTATTCCATTTATCTGAATGTCTGTATG11.9607843137254901No Hit
TAGTCATACTTTTCCCATCCCCCAGTCATCCCTGACTGTTGGCAACCACT11.9607843137254901No Hit
CCATTCTCTTATCTTTTTTTTTTTTTTTTTGAGAAAAGGTATCACTTTGT11.9607843137254901No Hit
GAGCCCACATTGCCAAGTCAATCCTAAGCCAAAAGAACACAGCTAGAGCC11.9607843137254901No Hit
GAAAAGCAAACAGGGTCCCTGAACAAATATACATTAGAGTCCAGCAAGAG11.9607843137254901No Hit
TCATTGAACGGAATCGAATGGAATCATCATCGGATGGAAACGAATGGAAT11.9607843137254901No Hit
GGCCTGGAGAGTGGGACTTTTCCAAGTGTTTGCCTTTCAAGTCATGTTTG11.9607843137254901No Hit
GTATGCATGTAGTGTACAGATGCAGTAGGAAATTCAAATATTGTTTTCTA11.9607843137254901No Hit
GTTGAGAGAAAGTGCTGTCCTTCATAGCTGTTGTAGTATAGGGTGAGTGT11.9607843137254901No Hit
CAAACAGAAGAACCCTATTTGGTCTTTTAACATAGTAACCTAGTTCTTTT11.9607843137254901No Hit
AAAATAAACAAAATCAATATAAAAAAAAAAAAACTTATAATAAATACATA11.9607843137254901No Hit
GTCTCTCAGGCCACAGTGCAATCAAACTAGAACTCAGGATTAAGACACTC11.9607843137254901No Hit
TATTCCCCTAGGATGTTACTAATAATATCACAGTGTGGACAATCACTGTG11.9607843137254901No Hit
GTGTCAAGAATGAGCTAAGAGCCTGTGGTAGATAACCGAGATGCTGAACA11.9607843137254901No Hit
CCCCCGCCCCAAAACTGGGCCTGTCCTGGGAGGCAGCCTGGCACCACTAA11.9607843137254901No Hit
GTTCTCGTTGGTTTCAAAGAACATCTTTATTTCTGCCTTCATTTCGTTAT11.9607843137254901No Hit
CTGAGAATGGTTACCTTCCCTCCAAACAGATACACTCCTTATTAAGTCTC11.9607843137254901No Hit
GCTCCGGCATCAAGGGGTCCAGGAGAAAATGCTTCCCCGTGATGCCGGAG11.9607843137254901No Hit
GCCCGAGACCGTTCTTACGGAAACACCCCAGGACACCGTCGAATGAAACG11.9607843137254901No Hit
TAGGGAAGCCCTGTCTCTACAAAAAAAATTTAAAGAAAAGTAGCCAGGGC11.9607843137254901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGT50.0145.06
GAGTGGG50.0145.09
TGGAGAG50.0145.05
ACATTAC50.0145.0145
GGCCTGG50.0145.01
AGAGTGG50.0145.08
CTGGAGA50.0145.04
GAGAGTG50.0145.07
GCCTGGA50.0145.02
CCTGGAG50.0145.03
Produced by FastQC (version 0.11.5)