FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18184978
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG75550.014.7784311
GTATATA322900.014.2800951
GTATAGA120100.013.6429241
GTATAAG118700.013.1319681
GTATTAG130400.012.9544921
GTGTATA211700.012.8083371
TATACTG166500.012.8020625
TATACCG14300.012.6750825
CTGTGCG34200.012.5075999
TCTATAC116550.012.5034033
TAATACT179550.012.4368314
TACACTG173200.012.4324125
GTATTAA176000.012.3580691
GTACTAT102500.012.2366981
GTCCTAG91850.012.2347361
ATAATAC192250.012.1432233
TATACAG167650.011.8925775
GTATTAT201800.011.71221
GTACTAG60250.011.5519751
GTCTTAG110850.011.5111581
Produced by FastQC (version 0.11.5)