FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32581
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGT100.0070596025145.06
ATCAGGC100.0070596025145.03
GTTAGAC100.0070596025145.03
CCCGTGG100.0070596025145.08
GTATAAG203.832176E-4108.751
GTCTTAT259.3047856E-487.01
TATATGC300.001918902272.53
ATGTTCC451.1766549E-464.444447
CTGAGGA350.003535592262.1428574
TAGCAGT350.003535592262.1428574
CCTGAGG400.00599863554.3753
GTATAGC450.00955620848.3333321
CCCCCCC300.001488914524.16666870-74
GGGGGGG14950.08.729097145
Produced by FastQC (version 0.11.5)