FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15849136
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA259300.014.5952781
CACGAGC29750.014.378044145
GTATTAG111550.013.3238171
GTATAAG101550.013.2079731
GTGTATA179350.013.0570641
GTATAGA103400.012.6911911
GTATAGG62800.012.4683351
TATACCG11057.2759576E-1212.4662865
TATACAG146600.012.4627055
GTATTAA149200.012.3912681
GTCCTAG81250.012.1355621
TATACTG133000.012.0472075
GTCTTAG93250.011.7401181
CCTATAC62100.011.6749263
TACACTG149600.011.4373695
GTCCTAT72500.011.4001671
GTATTAT164050.011.3579851
CTATACG14150.011.27228454
CTAGTAC52200.010.9724183
GTATAAT167250.010.9239261
Produced by FastQC (version 0.11.5)