FastQCFastQC Report
Fri 22 Nov 2024
HGWW3DRX5_n01_G3_5d_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWW3DRX5_n01_G3_5d_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28113429
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTTCCATCTCGTAT3942591.4023867383804371TruSeq Adapter, Index 7 (97% over 36bp)
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC823170.2928031297782992No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA754690.26844466393622773No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA720810.25639348369777304No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC720480.25627610207207385No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT714600.2541845749232511No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC678140.241215683792966No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG639580.2274998186809585No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC631670.22468621668313743No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT626020.22267650097040811No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA606260.2156478315042964No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT603860.2147941469537565No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC603690.2147336776314266No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC578580.20580200302140303No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC549420.1954297357323434No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT540830.19237425644520276No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA528820.18810227667354273No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG508280.18079615972850555No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA499460.17765886900527145No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA494270.17581277616472896No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT491880.17496264863314964No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG486370.1730027311858685No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT484890.17247629237970225No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG465920.16572862741147656No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG461890.16429514877036167No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC458040.16292569647053726No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC435940.15506468456764913No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG433310.15412918858101585No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA428890.15255698620043823No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA425020.15118041986269265No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC410340.14595871602855703No Hit
TCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGC407300.14487738226453983No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC403440.14350437294575485No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC403210.14342256150966146No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA399680.14216693381657572No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC398080.14159781078288244No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT394830.140441779620693No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC392780.1397125907337735No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT390460.1388873623349183No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA386150.1373542871629071No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA385780.13722267746136554No Hit
CCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAAC378340.13457625535469186No Hit
CACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACC378080.13448377286171673No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA377470.13426679470512115No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA375260.13348069351483235No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTTCCATCGCGTAT365070.12985609119399844TruSeq Adapter, Index 7 (97% over 36bp)
CCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTC364610.1296924683218116No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT362910.12908777509851255No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG362530.12895260837801037No Hit
GTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATT359090.12772899385556988No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT358400.12748355954728965No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC356550.12682551103958184No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA355250.12636309857470607No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC353290.1256659228584318No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA352590.12541693153119102No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG348810.1240723783640907No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT348800.12406882134513011No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT346300.12317956660498439No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA344090.12239346541469559No Hit
CTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAG339480.1207536796738669No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT338560.12042643392949327No Hit
AGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCC329980.11737451166131319No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT329930.11735672656651026No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG322810.11482412906657527No Hit
TTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAA321760.11445064207571407No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG318770.11338709340649979No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT310720.1105236931432306No Hit
GTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGC308190.10962376734620313No Hit
GCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGA307970.1095455129290703No Hit
CGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTT306840.10914356978652442No Hit
CCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCC300100.1067461390070916No Hit
CCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAG299580.10656117402114129No Hit
CACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCC297850.10594580974096046No Hit
CTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAG295470.10509923922834173No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC295110.10497118654576075No Hit
CTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGT291010.10351280877192177No Hit
CTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCG289100.10283341815045044No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA287710.10233899251492944No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC287180.10215047051001853No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA285920.10170228612098511No Hit
CTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGA283710.10091618493069628No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA283180.1007276629257854No Hit
GTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAAC281880.10026525046090962No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)