FastQCFastQC Report
Fri 22 Nov 2024
HGWW3DRX5_n01_G3_5d_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWW3DRX5_n01_G3_5d_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15490420
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCGTAT10063246.496428114925225TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCGTTT3895102.5145218786837282TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCGGAT3852862.4872534121089034TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCGGTT1956451.263006425907109TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCGTGT1183050.7637300989902146TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCGGGT664700.42910392358631977TruSeq Adapter, Index 9 (97% over 38bp)
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC490510.31665377697957836No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCGCGTAT474830.30653139166013577TruSeq Adapter, Index 9 (97% over 38bp)
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC426170.27511842803487574No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA374590.24182042836798484No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC306930.19814181926635946No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA298010.19238342149535No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCGCGGAT292970.18912979764267204TruSeq Adapter, Index 9 (97% over 38bp)
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT292190.18862626061785284No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT269610.1740495093096249No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCGCGTTT267400.17262282107263716TruSeq Adapter, Index 9 (97% over 38bp)
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG265710.17153182418552887No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC263660.17020842559465785No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA256910.165850893649107No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG256100.1653279898156409No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT253600.16371408909506652No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC247050.1594856692071616No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG236780.15285576504704199No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC231780.1496279636058932No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA225440.14553511137851652No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC224540.14495410711910975No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC224230.14475398342975854No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA223520.1442956356251154No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA218710.14119049063873026No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT215480.13910533090774815No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA211250.13637461088853625No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC208410.13454121966996374No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT204830.13223011383810124No Hit
CCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAAC204390.13194606731128014No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC197020.12718828798702683No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCGCGGTT196940.12713664316396844TruSeq Adapter, Index 9 (97% over 38bp)
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG196410.12679449621120667No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG196240.1266847509622076No Hit
CTCTGACTGGACTCGAACCAGTGACATACGGATTGAGAATCCGCCGTTCT195740.12636197081809272No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA193010.12459959123122549No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA192110.12401858697181872No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA191990.12394111973723114No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT191610.12369580682770384No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA188740.12184304880048444No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA187220.12086179716237519No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT186880.12064230666437709No Hit
TTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAA186780.12057775063555409No Hit
CACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACC186650.12049382779808424No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT178900.11549073556430363No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC171850.11093953553228382No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT171170.11050055453628758No Hit
CTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAG167920.10840248359954088No Hit
GTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATT165770.10701452897984691No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC164970.1064980807492631No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG164960.10649162514638079No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT164600.10625922344261807No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG159320.10285066512076496No Hit
CACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAA157250.10151435532412938No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT157050.1013852432664834No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC156010.10071386056672448No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA155330.10027487957072823No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)