FastQCFastQC Report
Fri 22 Nov 2024
HGWW3DRX5_n01_G3_4d_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWW3DRX5_n01_G3_4d_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10660373
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGTAGATCTCGTAT8680518.14278262120847TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGTAGATCGCGTAT331790.31123676441715503TruSeq Adapter, Index 1 (97% over 36bp)
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC304670.285796754016018No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC280080.26273001892147674No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA235380.22079902832668236No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT227380.2132946004797393No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT222340.2085668109361652No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA222260.20849176665769575No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC209020.196071938571005No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT202270.18974007757514677No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC198800.1864850319965352No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT191670.1797967106779472No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA185100.1736336993086452No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG180770.16957192773648727No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG172030.16137334031370196No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC171590.1609605967821201No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC165370.15512590413112187No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC164280.15410342583697587No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG163660.1535218326788378No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA162350.15229298261890087No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC155870.14621439606287698No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT153760.14423510321824573No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT152500.1430531558323522No Hit
TTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAA149940.14065173892133043No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC146230.13717156050731058No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC145170.13617722381759062No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG144980.13599899365622573No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA144680.13571757761196535No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG143590.13469509931781937No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA143580.1346857187830107No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA143050.13418855043815073No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA142780.1339352759983164No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA141430.13266890379914476No Hit
CCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAAC139600.1309522659291565No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA135240.12686235275257254No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA135210.12683421114814653No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT134020.12571792750591373No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA133430.12516447595220168No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC132760.12453598012002018No Hit
CACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACC131970.12379491787013458No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA128930.12094323528829619No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC126930.11906712832656043No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT125790.11799774735837104No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT124630.1169096053205643No Hit
CTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAG124440.1167313751591994No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC122920.11530553386828021No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG122230.11465827696648138No Hit
TCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGC120270.11281969214398033No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT119200.11181597491945168No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT119140.11175969171059962No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT118120.11080287716011437No Hit
CTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAG116100.10890800912876125No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA113990.10692871628413003No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC113700.10665668077467834No Hit
CACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAA112520.10554977766725424No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC112340.10538092804069801No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC112070.10512765360086368No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG111460.10455544097753428No Hit
CTCTGACTGGACTCGAACCAGTGACATACGGATTGAGAATCCGCCGTTCT110970.10409579477190901No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC109250.10248234278481624No Hit
CTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTT108390.10167561679126987No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG107490.10083136865848877No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT106940.10031543924401146No Hit
GTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATT106940.10031543924401146No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)