FastQCFastQC Report
Fri 22 Nov 2024
HGWW3DRX5_n01_G3_3d_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWW3DRX5_n01_G3_3d_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23453420
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCTTATCTCGTAT381385316.26139386068215TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCTTATCGCGTAT2360751.00656961756537TruSeq Adapter, Index 6 (97% over 36bp)
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC585720.24973756492656507No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT536760.2288621446253894No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC488560.2083107708811764No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA473800.20201744564332194No Hit
CTCTGACTGGACTCGAACCAGTGACATACGGATTGAGAATCCGCCGTTCT412580.17591464272587964No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA366470.15625439701331406No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT345380.14726210505759926No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC345330.14724078620516753No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG341360.14554806932208608No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA335580.14308360998097505No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT334230.14250800096531765No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT332470.1417575773597198No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA330880.14107963785238997No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC329850.1406404694922958No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA319710.13631700621913564No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC317920.13555379130207876No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA315390.1344750573690319No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC314650.13415953835304192No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG306030.13048416819380712No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA305550.13027950721046228No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC305120.13009616507954916No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA302690.1290600688513658No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG293260.1250393332827366No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA289890.12360244262883623No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC280310.11951775050291172No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC279210.11904873574941309No Hit
GTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATT274770.11715562165347314No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC271770.11587649050756776No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT264360.1127170365771815No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG256690.10944672461415009No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG254360.10845326609083025No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG253180.10795014117344079No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG252980.10786486576371379No Hit
CTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTG252460.10764314969842351No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT252280.10756640182966919No Hit
ATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTAT247220.10540893396357547No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT246000.10488875396424062No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC244460.10423213330934251No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC240580.10257779036063823No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC240430.10251383380334297No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT239960.10231343659048446No Hit
GGCTCTGACTGGACTCGAACCAGTGACATACGGATTGAGAATCCGCCGTT238820.10182736675504042No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC236320.10076142413345261No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC235950.10060366462545763No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)