FastQCFastQC Report
Fri 22 Nov 2024
HGWW3DRX5_n01_G3_2d_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWW3DRX5_n01_G3_2d_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13724759
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCCGGTATCTCGTAT137951510.051287603665756TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCCGGTATCGCGTAT1113550.8113439368953583TruSeq Adapter, Index 4 (97% over 38bp)
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT481470.35080397404428015No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT383910.27972075866687346No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC383720.27958232272056655No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA292460.21308935187860129No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC285120.2077413526896902No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA273700.19942062370639804No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC249330.1816643920669208No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC240910.17552949381479122No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC239040.1741669926590332No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC237890.17332909087875423No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC237850.17329994646900537No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA231330.16854940767994542No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA219360.15982794306260678No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA219030.15958750168217892No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC217140.1582104283215465No Hit
CTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCG214330.15616303353669087No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT210280.1532121620496214No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG205690.1498678410309427No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA199600.14543060464668267No Hit
TATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGC198640.14473113881271066No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT190680.13893140127269266No Hit
CTCTGACTGGACTCGAACCAGTGACATACGGATTGAGAATCCGCCGTTCT188330.13721916719994864No Hit
CGGCGACCACCGAGATCTACACTAGAGCGCACACTCTTTCCCTACACGAC188060.13702244243414402Illumina Single End PCR Primer 1 (95% over 23bp)
CTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTG186850.1361408240392418No Hit
ATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTAT186250.13570365789300928No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC184110.13414443197144663No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT179160.13053781126502842No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG174950.1274703621389636No Hit
TTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAA173080.12610786098320562No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA172530.12570712534915915No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC167680.12217336566711298No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT167430.12199121310618277No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG167340.1219256381842479No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT166850.12156861916482468No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT166580.12137189439902003No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA164770.12005310985788531No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG163260.11895290838986682No Hit
TGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGG162850.11865417818994126No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC161100.11737911026342976No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG157220.11455210251779284No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG157180.114522958108044No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC156790.1142388001129929No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA154940.11289087116210929No Hit
GCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCC153220.11163766154290942No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC152130.1108434763772537No Hit
CTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGC151420.11032616310421188No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG150990.11001286069941192No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA149350.10881793989970971No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA148230.10800189642674236No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC147180.10723685567083545No Hit
CTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTT147010.10711299192940292No Hit
CTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAG145610.10609293758819371No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT145450.10597635994919838No Hit
CCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACT145340.10589621282238908No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA144100.10499273612017523No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT142750.10400911229115208No Hit
CGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTA141120.10282147759388709No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT140780.10257375011102199No Hit
CGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTT139750.10182328155998951No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC139370.10154640966737558No Hit
CCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAAC139150.10138611541375699No Hit
CCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTG138320.10078136891146867No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC137470.10016205020430596No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)