FastQCFastQC Report
Fri 22 Nov 2024
HGWW3DRX5_n01_G3_1d_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWW3DRX5_n01_G3_1d_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16716069
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTGTATCTCGTAT6231333.727748431763472TruSeq Adapter, Index 8 (97% over 38bp)
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT522370.3124957189396622No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC520070.31111979736384193No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTGTATCGCGTAT517260.30943878013425286TruSeq Adapter, Index 8 (97% over 38bp)
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA420980.25184150651687304No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC386050.2309454453675682No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT353020.2111860150852452No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA325300.19460316896275073No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC312870.18716721018560045No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA297530.17799041150165149No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC279640.16728813454885835No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC276290.16528407486233757No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT271110.1621852601828815No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA270110.16158703341078576No Hit
ATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTAT270070.16156310433990193No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA266800.15960690279514878No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC265410.15877536758193567No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC258180.15445018801968333No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA246290.14733727169946473No Hit
CTCTGACTGGACTCGAACCAGTGACATACGGATTGAGAATCCGCCGTTCT243690.14578188209201576No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA242160.14486659513070926No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC235330.14078070627729522No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG227800.13627605868341414No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT225610.1349659420525244No Hit
TATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGC225420.13485227896582624No Hit
GCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCC225000.134601023721546No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG224390.1342361053905676No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA221320.1323995492002336No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT221110.1322739215780935No Hit
CTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTG220430.13186712737306838No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT218860.13092791134087803No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA217560.13015021653715356No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC216390.12945029121380153No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC215660.12901358567017163No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA213100.1274821251336065No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT212450.12709327773174423No Hit
CCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTG211130.1263036183925778No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC209820.12551994132113237No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG208010.12443715086363906No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA203110.12150583968036983No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT202110.12090761290827406No Hit
TAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCAC199910.1195915140096634No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC199670.11944793958436042No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC198650.11883774827682272No Hit
CTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCG197520.11816175202435453No Hit
GCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGG196470.117533613913654No Hit
CCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTC196020.11726441186621088No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT195000.11665422055867321No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC193010.11546374928220265No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG192910.11540392660499307No Hit
CTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAG191860.11477578849429253No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA190650.11405193410005665No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT189750.11351353000517049No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG189330.11326227476089025No Hit
GTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATT182970.10945755249036124No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG182250.10902682921445227No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC181640.10866191088347385No Hit
GCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCA179500.10738170559118894No Hit
TGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGG179020.10709455674058296No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG177820.10637668461406806No Hit
GTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGG175630.10506656798317834No Hit
CAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTT173860.10400770659656886No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT172350.10310438417070425No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG171400.10253606873721328No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT170990.102290795760654No Hit
CTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTT170750.10214722133535102No Hit
TAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTC170600.10205748731953668No Hit
GCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTA169470.10138149106706845No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA169330.10129773931897505No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC168720.10093282098799664No Hit
TTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCAC167730.10034057648362184No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)