FastQCFastQC Report
Thu 30 May 2019
HGWV2BGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWV2BGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422225257
Sequences flagged as poor quality0
Sequence length92
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8531810.20206773182211596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12604550.062.2475971
AGCAGTG13285200.058.9525072
AGTGGTA15302200.051.2708445
GCAGTGG15705250.049.9175643
CAGTGGT15737450.049.7220764
GTGGTAT16968800.046.2272076
GGTATCA17297450.045.1983228
TGGTATC17366300.045.0645837
GTATCAA17826900.043.7806139
ACGCAGA16917400.026.11789314-15
ATCAACG17206850.025.75347310-11
AGTACAT17368050.025.31308220-21
TACATGG17315200.025.20503222-23
AGAGTAC17816700.024.84513918-19
GCAGAGT18024500.024.62479216-17
CATGGGG10624050.023.85173424-25
GTACATG17315750.022.44314822-23
CAGAGTA17487650.022.4350618-19
TCAACGC17409200.022.4316512-13
CGCAGAG17735750.022.17217816-17
Produced by FastQC (version 0.11.5)