FastQCFastQC Report
Thu 30 May 2019
HGWV2BGXB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWV2BGXB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422225257
Sequences flagged as poor quality0
Sequence length8
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGCCT7218790717.09701298139064No Hit
TGTGCGGG7098194616.81139269221879No Hit
ACCATAAC6317085014.961409568163281No Hit
TGCCTCAT5658216713.400943231588819No Hit
CCAGGGCA5129192612.148000421490655No Hit
AATAATGG4665307511.049333081464617No Hit
GTGTCATC198398534.698878778822083No Hit
GGGGGGGG180110204.26573723418919No Hit
GTGTATTA87042172.061510261571112No Hit
AATAATGA4821400.11419023187426232No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)