FastQCFastQC Report
Thu 30 May 2019
HGWV2BGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWV2BGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422225257
Sequences flagged as poor quality0
Sequence length29
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC422500.016.51349423
ACAAAAG670150.016.4884593
AACGGGA673500.016.4743041
AGGGAGG808900.016.3696888
GGGACCT726950.016.3168321
AAATGGA780200.016.3015461
TGCGACG908150.016.1554991
GCCCGAA530700.016.1301361
GGGCCAT1009300.015.8755321
CCCGAAG934350.015.869911
TCTGCGA764250.015.8620348
CTGGACG617650.015.8614041
AAAGGGA592500.015.8505456
TACCGGG961650.015.7865971
AGATGAC627500.015.7160118
TGTACGA712300.015.3347817
GAACGAG619350.015.3009263
CACTAAG934500.015.1645271
CCGGACA564850.015.1549311
TGCGGAG683700.015.1256123
Produced by FastQC (version 0.11.5)