FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n02_daplib_ShaAmpPoolHm-input_ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n02_daplib_ShaAmpPoolHm-input_ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTAGAAGAAGGTTGGTTAGTGTTTTGGAGTCGAATATGACTTGATGT11.8181818181818181No Hit
GTTAGATAAACAGTTCGCATTCTTGAATTACTAAGTTACTACTGCATGTG11.8181818181818181No Hit
TTAGCTTATATCCCAAGCTGTCATCATTGCTTTTCTCTTGCTACTCAAAT11.8181818181818181No Hit
CTTCACAAGCAATGGTAACCTACAAAAGATATGATAAAGCATTAAGATAA11.8181818181818181No Hit
ATGATCTTACAAATGGACCAAGTCTTCTTGTTTCGGGAAGAAAAACGAAC11.8181818181818181No Hit
GTTATCTCGGTGGCGAGGTTCGTGATGAATTTTCAATTCTTTGTGTTTCT11.8181818181818181No Hit
CTGGGACTAGGTTAACTTTAGGGGCTCTATTGCGGTGGCACTAAGAACTG11.8181818181818181No Hit
ACTCAAATCTTTATGATTTACTACAACTAATCCAAAATTATTAAATATAA11.8181818181818181No Hit
AAACCAAAGATTACAAGACCAGCATAAGGCCCAAGTCCCAAGAGAGCTCC11.8181818181818181No Hit
TATCAAAAAAAATGAGTAGATAGAACACAACAAATAAAACAAATAATTTT11.8181818181818181No Hit
AAACTCAAGTCATGTGGTACTTATTGGACGCAACGAGAGATTGGCAGGAC11.8181818181818181No Hit
TGTTTCCTTGTTAGAAGACACAAAGCCAAAGACTCATATGGACTTTGGCT11.8181818181818181No Hit
ACTTAGGCTTATAAGATGCGGTTGCGGTTTAAGTTTTTATACTCAATCAT11.8181818181818181No Hit
TTTTTGTTATGCATGTATCAAATTTTATGAATTCAATAATGATTAAGTTT11.8181818181818181No Hit
TGTTTAGATCTACATTTTGATATGATTTCAGTAATTTCTGGGTTTGTGTT11.8181818181818181No Hit
TCTCAACCTTCTTCTTGGAAGGACATCCTAGATATGTGCACTTGTAATAG11.8181818181818181No Hit
ATCTTATAAGCCTAAGTAGTGTTTTCTTGTTAGAACATACAAAGCCAAAG11.8181818181818181No Hit
ACATCAAATCATATTCGACTCGAAAAAAACTAACCACGCTTTTTCTTGCT11.8181818181818181No Hit
AAAGCAAAAGACTAATATGGACATTTGCTACACAATGAAAGCTTTCAGAA11.8181818181818181No Hit
GGCTAAACCATGAAAGCTTTGAGAAGCTTGGTTAGTGTTTTGGAATCGAA11.8181818181818181No Hit
CTAGAAATCACCTGCGAATGCACCCCTGGTTGCGATGAAGGTCTCTCTTT11.8181818181818181No Hit
GAGATTTTGTGGTCAGCTTTGTGACGTCTGCGGCTAAGATTTCACCGGAG11.8181818181818181No Hit
AATTTAAACTTTCGCTAGCGTGATTGTAGAGTGTTAGTTTTTGACTTCCA11.8181818181818181No Hit
TGTATTGGGGGGGAGGGGTGAAAGAAAGTGTAGAAACAACATGTTTCTTC11.8181818181818181No Hit
AGTCTTGTGGTTATTTAATGTATTTTTGCCTAATGTAAACTCTATAGTAT11.8181818181818181No Hit
CAATTGTGTATATATGTTTCAAACCTGTAATAGCAATAAATTAGCCATAT11.8181818181818181No Hit
AGTCCATATGAGTCTTTGGCTTTGTGTCTTCTAACAAGGAAACACTACTT11.8181818181818181No Hit
AAAAATAAACAAAATTCCAAACTGTGTAAAAATAATGCACAATTCTGAAT11.8181818181818181No Hit
AGTTGGGTTTTGTCTGTGTTAACTTCTGTTTTGTGGGTGATTTTATTAGG11.8181818181818181No Hit
CAATATATCCACGGAGACGTACGCCCTATTCAGATATTCACGACCAAGAA11.8181818181818181No Hit
ATAGGAAAATGAATGATTTAGAAGAAAAAGCAAAAAAACAAGGCACACTA11.8181818181818181No Hit
AAAAATTTAGTACTTTGCTATTGATAAATTCTGATGTAGACAGTGCATGT11.8181818181818181No Hit
CAATGGTGTCTAATAAGTTTTTGTAACCAGCTTTTTTTATTTTATTGTTT11.8181818181818181No Hit
AAGGATCATTGTCGATACCTGTCCAAAACAGAACGACCCGCGAACCAAAG11.8181818181818181No Hit
TGAAGTAAACAAGTGTCTTAAAATTAACATCATAGGGTTGATCACTTACC11.8181818181818181No Hit
CCTGACCAACAACCTTCTAGGACTGAATTCCTAACTTCTTGTTTTAAGAT11.8181818181818181No Hit
CTCAAAGCTTTCATGGTGTAGCCAAAGTCAATATGAGTCTTTGGCTTTGT11.8181818181818181No Hit
AATACTGTAGCTTGCTGTATCCAGTGATTAATATTAACTACCCTTGTTGG11.8181818181818181No Hit
AGGTTAGTGTTTTGAAGTCGAATATGACTTGATGTCATGTGTATGATTGA11.8181818181818181No Hit
CATCCATTGTCTACGATGATAGATAGACAAAGGATGAGAATTCCATACAC11.8181818181818181No Hit
AATGTGGTGTGGGAAGTGCAATCTCTGTTTAGGGCTAAGCTTCCTCATCT11.8181818181818181No Hit
GTTTGGAGATACAAAGCCAAAGACTCATACGGACTTGGCTACACCATCAA11.8181818181818181No Hit
GATTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGCGCCGAAGC11.8181818181818181No Hit
CGTTTTTCTCGACTTTGGAGAAGAGGGAGAGGAGAGAGTGGAACTGCTTT11.8181818181818181No Hit
GCGGAAAAAGTTCTTATACTCAATAATACACAAGACATACAGTCATATTC11.8181818181818181No Hit
AGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTCGAATATTACATGATGTA11.8181818181818181No Hit
TTTTGTCAAATCCTAAAGGAACTTATGCTCTATTTATGTATTGAATCTAT11.8181818181818181No Hit
AAGATGGTCGCTTACAGAATAGGCTTCAGAAACAGGGGATCTATCAAAAA11.8181818181818181No Hit
ATTGTACTATGCTTTTTTGTTAATAAGCATACGGTTTTCGATATTGAAAA11.8181818181818181No Hit
GAACAGACAAGAGACCAACTCTCTGGGACTATTCATAAAAGACACAAGAA11.8181818181818181No Hit
AAACAATTAGGTAATAAAACTCATCACGCTTTTGTTAAAACATCACAAAA11.8181818181818181No Hit
TTAATGTGATTCATATTGTAATAAGCTTGGCTAATAGCAAGGAAGTTAAA11.8181818181818181No Hit
GGAACCACCAAGACTAAGAGACAAAACAAGCCTAAGCTCTCTGTTAAGAT11.8181818181818181No Hit
AAATGAATACAGAATCAAATTTTCTGATACAGAATGAGTAAGAAAAAGAT11.8181818181818181No Hit
GGAGTGAATAGCCAAGAAGGTGCCTCGATCAATGACCAAGGGTTAAGTGG11.8181818181818181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)