FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n02_daplib_LerAmpPoolHm-input_ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n02_daplib_LerAmpPoolHm-input_ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTACGTGCACATGTAACATACCATACAGAAGCATCGTTAGATCCATCA15.88235294117647No Hit
ACATAAGCACATTTTAATTGTGGAGTTCATACCGGATATTTGACCGAAAA15.88235294117647No Hit
CTTTATTGAAGCATCTGTCACGCTAAATGTTCAAATCGCCGTGTAGGTTG15.88235294117647No Hit
ATCTCAGCCACAAGTAAACGGTACGCCTGGACCATGATCACTGGGTTGCT15.88235294117647No Hit
AAAATTTCCTAAAGGTAACATAATTGTTGCACATAAATGTTGGATCTGCA15.88235294117647No Hit
TCTAGGGTTCCATACTTTGGGACTTTATGTTCATAACGACTTCATGCGTG15.88235294117647No Hit
AATTATGTTTTGTTGTGTGGGCTGGCTGGAGGCGGGGTGTGCGCTGTAGG15.88235294117647No Hit
ACTTCTTCTTCGTCGAACTTGATGTTTCTTTTGTGATCATCTTCGTTATG15.88235294117647No Hit
GCGAGAAGAAGCTTGGTTAGTGTTTTGGAGTCGAATATGACTTGATGGAA15.88235294117647No Hit
GCAACCGAATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAAGATCGG15.88235294117647No Hit
ATTGTATCCTTGTTAGAAGACACAAAGCCAAAGACTCATATGTACTTTGG15.88235294117647No Hit
AAGCCTGTGTTATTGCAGTGGACGTGATCAATGGTTAATGAACAAGGAAG15.88235294117647No Hit
ATAAAGACTTTACCTTCAGTTCAAATCGAATTTCAAGCCAAATGGATAAA15.88235294117647No Hit
TGTTTCCAGTTAATAGTGGAGTCTTACTTACTTGAATTCCTATTACTGTT15.88235294117647No Hit
CGTATCTTTCATTATAGTTCTATGATTCAGAGTATCATTTCCTATTAGAT15.88235294117647No Hit
AAATTGCAACATTTAACCAACCATATGGAGTAGCAAAGGCCGGTCCGGTC15.88235294117647No Hit
ACGCCACGGAAATCGATATCTTGTTACCGGTAGCGTAGATCTCAGGTGAT15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)