FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n02_daplib_EriAmpPoolHm-input_ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n02_daplib_EriAmpPoolHm-input_ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11.2987012987012987No Hit
AGAATCCCTTCAACGCCTGCACGAGAAAGAATGTCTACGCGTTGCCTTCG11.2987012987012987No Hit
AAGATGTTTGGTTAGAGATGTCGAGTCGAAAAAAACATCGTCACAACAGT11.2987012987012987No Hit
TGGTAGATAAGTTTGAGTTGCATAGTGGCAGTCTTCCTTTGTTTGTTAAC11.2987012987012987No Hit
AATAAATCAAAAAAATTTTCTGCTTTTAAACTCTTATTTGGATCAAAGGA11.2987012987012987No Hit
AAACTCCTTTACTAATACCATTGCTTATACAAAAATGGCTGCACTTGCTT11.2987012987012987No Hit
GATAAGTTATCAGAAGGAGAAGCTGCTGATTGTTTAGCAGCAGCGAGAGG11.2987012987012987No Hit
AACAAGGAAAGACTACTTAGGCTTTTAAGATCGGGTTGTGGTTTAAGTTG11.2987012987012987No Hit
ACACATGACATCAAGACATAATCAACTCCAAAACACTAACCAAAATTCTT11.2987012987012987No Hit
AAGAAAGCCTTTTCACGAGGGGTGTTTGACTCTGAAAAGCACGATAACCA11.2987012987012987No Hit
TACACTTAAAAAAAAGGAACAAAATGGATTAAACAAATGATGAAAGGAAC11.2987012987012987No Hit
CTGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGT11.2987012987012987No Hit
GGCCTACAAGTTTGGTGTCTAATCAGTGGTAAGCAGTAAGCAATAAGCAC11.2987012987012987No Hit
CAGCGAGAAAATGGGGAAATCCCATAAAGCCCGATATATCTGACAAATCG11.2987012987012987No Hit
ATAAATCACGAAAAGCAGACATGAGAAAACAAATCCAGTCTTTCACTAAG11.2987012987012987No Hit
AACTTGCTTAGTTCGGGAAGACAGACTTCCATTAACTTGTTTAGTTACCT11.2987012987012987No Hit
AAACAAAGAAATTTTGCATTGAGAACTGAAAAATGAGGCACGCAATGCGA11.2987012987012987No Hit
AACTTATTTATTCTCACAACTTTAATTCACCAACAAAGATATACGTACAT11.2987012987012987No Hit
ATTGGACTTGATGTGATGAGTATGAGTGAGTAGAACAACGTAAACCGCAA11.2987012987012987No Hit
GATATGTGTATTTGATATCGTCGTGTTTATTGATTGGTTGATGTTGAAAT11.2987012987012987No Hit
CTAACCCAATAACGAAAGTTTCGTAAGGGGACTGGAGCAGGCTACCACGA11.2987012987012987No Hit
ACATGACATCAAGTCATATTCAACTCCAAAACACGAACCAACCTTCTTCT11.2987012987012987No Hit
CCTGCTTGCACTTACTTTGGCACGAGCCAGTTCCCGATCAAGGATTCTCC11.2987012987012987No Hit
AGTTTAGGATGTCAAGTTTGCATCAAATATGCCCACGGAGCCCCAACTAG11.2987012987012987No Hit
AGAAGGAGTGGTACAACATTATCCATATAATAAAAGAAGTAGCTAATGGA11.2987012987012987No Hit
ATTATGCCACGAAGATACAAATAGTGAAGACGCAAATATGATGTGTGCTA11.2987012987012987No Hit
CATTTCACAACGTTTGGATGATCAACACTTCTTAAGATCTCGAACTCTCT11.2987012987012987No Hit
ATATGTTGAAACTATAAATAATGGTAAACTATGTTAGAGCCAACTGTTTA11.2987012987012987No Hit
ATACAAGAACAAGCTAAACCAAACTCTATCCTTCTTGATACCTCTTCTAA11.2987012987012987No Hit
ACGCAAAACATGAACCTTAAACCTTAAACCCTAAATCCTACTAGATTGGA11.2987012987012987No Hit
CAACACCGGATCTTGAAGCCTCTAAGGGTAGTTATCCGACGTATCTCGGC11.2987012987012987No Hit
ATCGTCACGCGCCACGGTGGCCGCACCTGGGCCGAGGGCGAGCCGGACGA11.2987012987012987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2987012987012987No Hit
TTTGCCCTGAACTGGTGAAAGGAATAGGAGGACTTTTCCCTCGGTGGAAG11.2987012987012987No Hit
AAATGAAATTAATGTGATCAGACCTCGACTAGGCCTAGATTGTCATAGTA11.2987012987012987No Hit
GTCATGTCTTGAGCTGATTGCCAACACTTCGCTTCAGCCTGAGCTTTCTC11.2987012987012987No Hit
GAGGATAAGAAAGTCGCTGTGTGTTGTGAGAGATGGTTGGAACCGCAAGA11.2987012987012987No Hit
TCCATTATCAAACTGAAAAACCTAAAAATTGATTATAACCTCACTGCATT11.2987012987012987No Hit
ATGAGAGGGGAATGAGGAGGATCCGAAGGCGCATTGAACGATCAACAAGA11.2987012987012987No Hit
AAATCCTCCCGGTTCATCAATGTGTACCGAGCGATCTCTCTCAGAACTAA11.2987012987012987No Hit
AGAAGACACAAAGCCAAAGACTCATATGGACTTTGGATACACCATGAAAG11.2987012987012987No Hit
TAAAGCTTGATAAGAGATTTACCTAGTGCTGTCTAAGACAGAGAAGGAAG11.2987012987012987No Hit
TCTTAAAGGCGTAAGAATTGTATCCTTGTTAAAAAGACACAAAGCCAAGG11.2987012987012987No Hit
TATAACTCTTACGATAAGTCAAATGCATTGGATTGTGACACATTTTGACC11.2987012987012987No Hit
GACTCTCATAGAAAGAAAGGGTAAGCTCTCTGCTCTCTATTTGCTCGCAA11.2987012987012987No Hit
CAAAAATAAAAGGATATACGATTAAACTAGTTTATTTTACCGCAATAAAT11.2987012987012987No Hit
AAGTGTCATTGTGTCCTCATCAAAAGGTTACGTATTCGGACTATTGCGAT11.2987012987012987No Hit
GTGATGAATGGCTACATATAATATGTGACGCTCATTTAATATTGAGGTTC11.2987012987012987No Hit
TCAATTCCTGCGCCTGATTTCTGATTCTTCACCTCCGAACCTCCTCCTCC11.2987012987012987No Hit
ACCATACCGCCTTGGAGGATATATGATTGCATTTGTTTGTCACTGCACAT11.2987012987012987No Hit
AGAAATCCTTTGTTGCAGGCCGCGAATGCGGTCCCGTAAGACTTGCATCC11.2987012987012987No Hit
AGTTTCCTACGGCGAGCTCGAGCAGAAGGCGCAGCCGAAGGCGCAGCCCT11.2987012987012987No Hit
ACTAACACGACTCATTCACATCAAGATCATGAATGTATGGAATCCATATT11.2987012987012987No Hit
TAGATTTCTTTACTGTAGTCACCATCCGAGAAATGAACTCTAGAATTTAC11.2987012987012987No Hit
AAGCAAGTTTGGGTGAAATTACATAAGCCAGGAAATATGCGTTGAAGAAA11.2987012987012987No Hit
AGACTAGTATGGTCGTGGTGGAAAATTCTAGACATAAGGTGGTGGAGGAG11.2987012987012987No Hit
AAAAAAATAATAACAATTAACATGTCACCTTCATATCATTGAAAACAAAC11.2987012987012987No Hit
ATAGATAAATCAAAAAGTTCGTCTACTAAATACTCAATTACTAACCAAAT11.2987012987012987No Hit
TTTACACTAAACGTTTTTTAATTTGAGTAGTTTTATCTTTTGTAGCAACT11.2987012987012987No Hit
ATCAACACTCACCATGGAATATTAAATAACAGATCATATACTTTCTAACT11.2987012987012987No Hit
AACTTTTCCATTGACTTTTTGTTGACTCCAAATTTTGACCTTTAAGTACT11.2987012987012987No Hit
AGAAAATTCGAATTTCATCTTCCGAGTAAGAGACTAAAAGATGAGTTGTC11.2987012987012987No Hit
AAAGCCTAAGACTCATATGGACTTTGGCTACACCATGAAAGCTTTGAGAA11.2987012987012987No Hit
AAAATAGAGAGAGATTAATAAAAGCAATAAGAGTTAGTAATTGAAGACCT11.2987012987012987No Hit
CTGATAATCTCTTTGGTACTGATTGTATCCTTGGTGCTACTGTACTTCAA11.2987012987012987No Hit
GACTCGAAAACACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGG11.2987012987012987No Hit
CATGAAAGCTTTGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTTG11.2987012987012987No Hit
AAAATGAGTTTGCATCAGATAATGCTTTTTGATAAAGGGGTTTCAAAAGT11.2987012987012987No Hit
AATAGGAACCGGTTTTCATGATTCAGAAAAACTCGGTGGTGGAAGCATGA11.2987012987012987No Hit
ACAAAAGTCCCGCTTTCCCAGGGACTTCTGTCACCACAGACACAATATAA11.2987012987012987No Hit
GTGAAGGGAGAAGAAGTGGAGGCGGAATGTTGCCGTTGCTGGCTCTAAGC11.2987012987012987No Hit
AAGGCATGCATATTGCAATAATAATGTGCGATTTGTCAAACAAGTGGGGC11.2987012987012987No Hit
AACGATACAATTCTTACGCCTTTAAGATCCGGTTGCGGTTTAAGTTCTTT11.2987012987012987No Hit
CATATATAAAATGCAAGGTTATACATAAAATTAACTCTGGTCACCAAAAA11.2987012987012987No Hit
GCAAATGAATCGGTTGTTGAATTAGCCTCTCGCAGCATTTAAAAACGCTG11.2987012987012987No Hit
TAAGTAGTTTCTAGTACTAACCATTGCCACTTATGTAGTCTTTTTGATCT11.2987012987012987No Hit
TAGTGGTATCAGCGAACCTAGACCACACAGTCATCATACTCTAACGTGTG11.2987012987012987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)