FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n02_daplib_EriAmpPoolHm-input_ac5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n02_daplib_EriAmpPoolHm-input_ac5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTTACTGATTAGTGTACCCGTTGTATTTGCGTCTCCTGATGGTTGGT18.333333333333332No Hit
CACGATTAGGGCTTCCAACGGCAATCTCCGGTAAAGTTCATCAGAGATGT18.333333333333332No Hit
CACCAATCTCCTCGACGATTCAGCAGTTTTTGTCCGAGAACTGCTGAGAA18.333333333333332No Hit
AGTTTTCATTCTTGTTAATGATTAGACTTGTCTTTATCTTGATGATTCTT18.333333333333332No Hit
CCATCCGACAAGACCAGAGAAAGGACCAACACACATGAGAAGCCCAGAAG18.333333333333332No Hit
CTTTTCCTCTTTCCTCTCCATATTCTTCTCCTGATTTTCCTCTTTTCATT18.333333333333332No Hit
ATGCCGCCAGGCGTCTATTATTGAATTCCCCCGGCCCGAGAGTAGATCGG18.333333333333332No Hit
AATAGGAAACAATCACGCAGCAGGAGCATAATCATAGCCACTGTCATCGT18.333333333333332No Hit
TCTGTAAACAAATTTTAGTTCGGGTTGATTACGTTTCGATTTGGTTTGGT18.333333333333332No Hit
AAAAATCGTAGTTCAGATTCAAGTCGGTTCAGTGCCGAGGCACTGTTAGG18.333333333333332No Hit
CGAATCTTGGGATATGGAATGAAACTGACTTGTTGAGCTTGAGCAGCAAG18.333333333333332No Hit
CATTAGGTCAGATAGACAGTGATAGGTCGTCACAATGGAGCAATAGTATG18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)