FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n02_daplib_CviAmpPoolHm-input_ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n02_daplib_CviAmpPoolHm-input_ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCAAGTTTGATATTCATTCAGTATATGTAGCAACCTCACATAAGTCA12.941176470588235No Hit
GGTCATAGGCTTCAAAACTGACCTTCATAGCTACAATTGCGAAGAAGTGC12.941176470588235No Hit
AGAATCTGACCCATTTTCTCATTGAGCGAAGGGGTACGAAATAGATCGGA12.941176470588235No Hit
TTTCTTTCCTTTTCGTTTGTTTCGAGAAAGAGATCGATCAATTCCGATTT12.941176470588235No Hit
TGCATATATAGCAGATACAAAGCAAATTAATCATACGGACTGTGGATACA12.941176470588235No Hit
TATTTCGTCGGAGGGCTGTCTTTGGGCTTTCCGAAAAGGTATCACATGCC12.941176470588235No Hit
TACATAGGCTAAACCATGAAAGCTTTGAGAAGTAAGAAGAAGGTAGGTTA12.941176470588235No Hit
AATCTTTAACGTGTAGTTTCATTCATCTAGCTCGTTTACTTTTGTCTACT12.941176470588235No Hit
AAAATGGATTTAACCCTGAGGTAATCTAAAGATTGCTTAACTGGTTGGAA12.941176470588235No Hit
CTAAATTACCTTTTAGATTGATGAACATTTTCACCTGTAGGGCATCTTAA12.941176470588235No Hit
CTTCTTCAACCTCATATTATCCAGATTTTTGCACATGCACGGCCTATTCT12.941176470588235No Hit
TTGTCAAGCATCTCCTCCAAGTATCTGCATGCCTCTCTCGACTTCCCTTC12.941176470588235No Hit
CATCGTAAATCGATCCTTTATAAGCCATCCCTTTCACAGAATTGAACGTA12.941176470588235No Hit
ATTGCAGAGATACCAAGAGATCTTTGACGTTGTCTTGAGCTGTGAAGCTA12.941176470588235No Hit
GCTCTTGTTGTCTATGTTCAGTTCTATGGAATGAAGGTCGCTTTCGCCTG12.941176470588235No Hit
TCATCATCCTCATCCTCACCATTACCATCGTCTTCATTTTCACCTTCTAT12.941176470588235No Hit
AACGTGAGGCCAAACTTGGCATGTGGTACCTTTTAGGAAAGCCCAAAGAT12.941176470588235No Hit
ATAGGGAATGATGTAGTGTCCATTAATCCATATCTATTTCTAAATTTAAT12.941176470588235No Hit
GCATGAACCACATCATCTCACCTCTTTACACCTAGGATCCTCAGGAATCA12.941176470588235No Hit
AGTTGGAACATGAAAACGTGACCGAGTTTGTCCTAGTTACTCTTCGGGAC12.941176470588235No Hit
AAGATTGGGTTGCGGTTTAAGTTCTTTTACTCAAGTATACACATGACATC12.941176470588235No Hit
AGAGATATTCATAATTTTCCATCATTTTGGTACGTTCTCATTATTACACA12.941176470588235No Hit
AAATAAATATGATGGAAGTGAAAGAGAAGAGTAGATTATATTTTATAACA12.941176470588235No Hit
ATACTCATATGTACTTTCGCTAAACCATGAAAGCTTTGAAAAGCAAGAAG12.941176470588235No Hit
TAACCATGTTATTGTTTTTATTTGTACCAAATCAAGATTTCTATCACACG12.941176470588235No Hit
TCATGTATATAAATTGACATTGGTTACTAAACAGCATCCGGACAATAGCG12.941176470588235No Hit
TATCTGAACTTGATAATTTTGGAGTAGAAGAGCTGGAAGAGATATTAATA12.941176470588235No Hit
AACTCCTTAGCATCTTCCAAAGGTACCACTGTATAATCCGAACCATGAAC12.941176470588235No Hit
CCCATGTCCTTAGGATATGACACCAACAAATTTGCAGGATTCGGTCCTGC12.941176470588235No Hit
TAGATCGTGCGACTTTGAAATCTGATGGTGTTTTTCGTAGCAGCCCAATA12.941176470588235No Hit
ATTAATGTTTTAATAAAAATTGTTAATGACGCAAGCTGCGATTTGAGCTG12.941176470588235No Hit
TTTTATATACAGTCTCTCAAGTCATAAAGCTCTAACAAAGTTTCTAACGT12.941176470588235No Hit
ACATAAACCATGGAAGAGTTGACAATGTTTACTGGATTTTAGTCAGAGGA12.941176470588235No Hit
TCTTAATGGTAACACAAGTAATCAGACTTCTTGCCTGCCATTACACAATT12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)