FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n02_daplib_An1AmpPoolHm-input_ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n02_daplib_An1AmpPoolHm-input_ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGATTTGCACATATAGGACAAATGAACCAAATACCGCGTCTTTTTTTA12.2222222222222223No Hit
AAGAAATTAAACCACTTTAAGGTTAGCAGCAAGAGATGAGATAAAAGAAG12.2222222222222223No Hit
TTATACTCAATCATACACATGAGATCAAGTCATATTCGACTACAAAACAC12.2222222222222223No Hit
ACCAAACTATAACCAATAGTAAATAGAGAAGAACACATGACTATACAAAT12.2222222222222223No Hit
TAGGTATACAATCGTTATACCTACTAATGAAGATATTTAAACAGCATCTC12.2222222222222223No Hit
TAAGACAAAGAGATCTTTTCTAAACTCGGACATTCTATCTTCCTCCTCCA12.2222222222222223No Hit
CAAGACTTGACTGCTTACATACACTCGACTGCGTCACCACACCGGACAAG12.2222222222222223No Hit
AGTAGTGAAATGATGGGTTTGATGGGAAATACCATGTAACATTGAAGTTA12.2222222222222223No Hit
ACTCGAAATCTTTGCTACCCGTCTCGTTCCAGTTCCTAGCCGCAAGGTGT12.2222222222222223No Hit
AGATAATAAAATTCACTACTAGGAAACATACCGGTGATGCGGACGAGAGT12.2222222222222223No Hit
GCAGACAATTTGGTAAACGAGCGTTTGGTTCTCTGAGTTCATCAAATAAC12.2222222222222223No Hit
TTCTTGCTTCTCAAAGCTTTCATGGTGTAGCCAAAGTCCATATGAGTCTT12.2222222222222223No Hit
AAGTCTACTTATCACGACACAAAGGACAAGAAGTACTTATTCATGTGCAT12.2222222222222223No Hit
AGAATAGATATTGAAGCACATACACTGATGGAGAATCTAGATAAGACTAA12.2222222222222223No Hit
TACCACGAGAGGTGTTAGCAGCTGCCGATCATTAGATTGCCATGAAATTA12.2222222222222223No Hit
GTTGAAGCTCCAAAAGGGGAGTTGGGAATTTTTCTCATAGGAGATCAAAG12.2222222222222223No Hit
CAAATTAAAAGACGAAAATAAATAAAGTCAGCAAAATAATAATAGATGTA12.2222222222222223No Hit
ACCCGATCTTATAAGCCTAAGTAGTGTGTCCTTGTTAGAAGACATAAAGC12.2222222222222223No Hit
CCATGAAAGCTTTGAGAAGCAAGAAGAACGTTGGTTAGTGTTTTGGAGTC12.2222222222222223No Hit
CCTGGTTTATATCCCGGAGTACTCCTATGGCCGATCTGTCACCCAACCTA12.2222222222222223No Hit
AATTAGGATACATTTGGCGGGTTGCATTTTATACTGGATAGCCTACCATT12.2222222222222223No Hit
ATCCATCATTTGATGCTAGTTCTTAGCTTTTACAACTGTCTTAGCTTGAG12.2222222222222223No Hit
AACAGGATGTCAGAATTTTGGGATCCAGGACATGAATGTTGATGAAGAAA12.2222222222222223No Hit
CCTGACATCAAAAAGGAAGAAAATATTAGCTTAACAGCGATTTCACACTT12.2222222222222223No Hit
AGACTTGTGTAACTATGCAATCCCAATCAACAATGATAATTTAAAAGTAT12.2222222222222223No Hit
GCCTTTAAGATCCGGTTGCGGTTTAAATTCTTATTCTCAATCATACACAT12.2222222222222223No Hit
GAAAGCTTTGAGAAGCAAGAAGAACCTTGGTTAGTGTTTTGGAGTCGAAT12.2222222222222223No Hit
ACACGCTTACGCGTGGAGCTGAGGTGAGAGAAGGTTGAGATTAACGACGA12.2222222222222223No Hit
TATTGTTGATCATTTTTAGGTTATATAGGAAGCTTTGGAAGCACATGAAG12.2222222222222223No Hit
ATAAAACTATATACTTAATATCCCGAAAGTATGTTTACATTTAGTACTCG12.2222222222222223No Hit
ACATCATTGTTCAGATCAAACAGAAGACGCAGAGTCGCAGATGCCAAGAT12.2222222222222223No Hit
TTAATGTGGATAACTAGTGTTGTAGTATTCGTAATCTATCAATTATCCCA12.2222222222222223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.2222222222222223No Hit
AGTAGCTTAGTTTGGCATCAAAGATAAAGTTAGTTTCCGATAGAGTCTCC12.2222222222222223No Hit
GGAGCACCCGTGTCACGACCACTGACAACCAAATTGCCAACCCCGTTCTC12.2222222222222223No Hit
ATACCTGATGGCTGCAGAAGTAGCGATGGATTTCTAAATACGTCTCTTTT12.2222222222222223No Hit
CCGAATAAGAACTAAAGATAACTAAAATATAAATATTTTAAATACAAAAA12.2222222222222223No Hit
AAGATGTAGATGCCTTGAAAAGATATAGCAGCTCTAATGTTTCCTTGACT12.2222222222222223No Hit
CAAAGATGTTTATTCACACAACCTATCTGTACTCAGCGAGGAAGAAAAGA12.2222222222222223No Hit
TACTACTGAAGCTTTCTGTCCTATCTGCAATGGCCCGCTAAATAGATCAG12.2222222222222223No Hit
AAGCTTTGGCACAGAGGACACCACATCCAAAACTAGGAAGAAATGAGCTA12.2222222222222223No Hit
CCAAAATACTCTTCGGAAATATCTCTTAAAAACATTATACAACTGATACG12.2222222222222223No Hit
CATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGATT12.2222222222222223No Hit
TAAAAACCATGAGACTGTCTTCTCTGCCGAGCAACACTCTCATAAATCTC12.2222222222222223No Hit
GAAGAGCCGAAGATCTTTCTCTTGATCTGCATCCACCACCGGCACACGTA12.2222222222222223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)