FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11465581
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG3886803.389972126139966TruSeq Adapter, Index 6 (97% over 36bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302510.26384184107198755No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG228390.19919618552256532TruSeq Adapter, Index 3 (97% over 34bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183680.16020121439986337No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT181870.15862257656197276TruSeq Adapter, Index 6 (97% over 36bp)
AAGGTATGCGCTTTGACAAGGCTGTCTCTTATACACATCTCCGAGCCCAC120240.10487039426959696No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)