FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n01_daplib_ShaAmpPoolHm-input_ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n01_daplib_ShaAmpPoolHm-input_ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTAAATTGGTACTTAACTCTATCACTTCTCTCTTTTGATTTTAGATC11.8181818181818181No Hit
AGTCTTATAAGATCGGGTTTCTGTTTAAGTTCTAATACTCAATCATACAC11.8181818181818181No Hit
AGTATGGGCAAAAGAAAGTTAAAGGCAGCAAGTTTCCTCTAAGCTATTAC11.8181818181818181No Hit
AAGAATTGACAATCCCAATTACTTTCAATTCCAAATCAATACTCAGAATT11.8181818181818181No Hit
ACAAGGGCAGAAAACACCATCAGCCGGAAGGAAAGTCCTTTCAATACCGG11.8181818181818181No Hit
ATCAAAATCACAACCACTTAACCCTTGGTCATTGATCGAGGCACCTTCTT11.8181818181818181No Hit
AAACTTTGCATTTAAGAATTCTTACTTCGTTTACTTCTATGGATTCTTAT11.8181818181818181No Hit
AACAGGTTGTGGTGTGTTCTAGCAGTTCTTAGTGCCACCGCAATAGAGAC11.8181818181818181No Hit
TGTAACTAGCTGACGTGTAAAAAAAAAGAATGTATAAAAAAAATAGGATC11.8181818181818181No Hit
GATGAAAATCTACATTTGATAAGTACGTAAGAGAAGATCTCATCTATGCG11.8181818181818181No Hit
GTGCCGGAAGACCTCAATATGACCAACAAGCGGACGGAATAATAATCACT11.8181818181818181No Hit
GAAGAGGAGGAGGAGCCAGCCACTACGAACTTCCGCGCAGCAATACGGTA11.8181818181818181No Hit
TATTCGACTCCAAAACACTAACACACATTATTCTTGCTTCTCAAAGCTTT11.8181818181818181No Hit
TAATGTAGGACAATAACTGCTAGTCTTCACAGGGTTAAGCGAAGGAAGAG11.8181818181818181No Hit
AGGCTTTTAAGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATACAC11.8181818181818181No Hit
GTCAAAAGCACTTCCACTCTCTCCTCTCCCTCATCTCCAAAGTCGAGAAA11.8181818181818181No Hit
TTTTTCAAGTAGTATTATGTAGTGTTTGCAGATGTGAACTACTTTCTATA11.8181818181818181No Hit
AACACTAACCAAGCTTCTTCTTACTTCTCAAAGCTTTGATGGTGTAGCCA11.8181818181818181No Hit
ATTTACAACGGATTTTAAAAGGTTAATTAAGAATTGTGCATTATTTTTAC11.8181818181818181No Hit
TTGGCTTTGTGTCTTCTAACAAGGAAACACTACTTAGGCTTTTAAGATCG11.8181818181818181No Hit
AGAAAAGGGAAAGAAATCTGGATAATGTTAGTAGTCCGTACCCGTACAAC11.8181818181818181No Hit
ATAGACTTTGGCTACACCATTAAAGCTTTGAGAAGAAAGAAGAATGTTGG11.8181818181818181No Hit
GGTTTAAGTTCTTATACTCAATCATACACATGACATCAAGACATATTCTA11.8181818181818181No Hit
TATTACTAATTATTTTTTTGTAATCTATACATATAATTTAAAGACTACTG11.8181818181818181No Hit
AATGGATGAGGCCGGAAGTTCAAGAAGGATGTGATGTTTCTGACTGGTTC11.8181818181818181No Hit
AGAGAACGGTTGTTGAGATGATTACATAACTATAAAACTAGAAGCAACCA11.8181818181818181No Hit
ATAGACGCCTGGCGGCATTCCAGCCTTCCTTCTCCTTTCAGGACCTATCC11.8181818181818181No Hit
GGATTTGGTCAAAGTAAGCCCATCTAACAAATCCCAGCCGTTGGGTTTGG11.8181818181818181No Hit
TGTTGCTTGGGTTCGTTTTTCTACCCGACACAAGAAGCCTTGGTCCATTT11.8181818181818181No Hit
GGTACTAACTTAAACACTAACCACCTAACTAACCTAGAGATTACCCTAAT11.8181818181818181No Hit
CTAAAATACAAACTAACCGCTAACTATTCAATCGATAAGGTGAAATATTA11.8181818181818181No Hit
CCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGAGAGGTG11.8181818181818181No Hit
TAACAGAGAGCTTAGGCTTGTTTTGTCTCTTAGTCTTGGTGGTTCCAGAT11.8181818181818181No Hit
GTTTCCACCAAACTAAGACCACAAATCCCTACATGACCTTCAAAGGATGA11.8181818181818181No Hit
TTTTTTACTTAGCGAAAGATAACTTTCAATTTTCAATATCGAAAACCGTA11.8181818181818181No Hit
ATCGAAATAACCACAGGTTTCTAATGTACAAACTCAATAAATATTGTATA11.8181818181818181No Hit
ATGTGACTTGATGTCATGTGTATTATTGAGTATAACAACTTAAACAGCAA11.8181818181818181No Hit
CATATGGACTTTGGCTAAACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT11.8181818181818181No Hit
TAGGCTTATAAGATGCGGTTGCGGTTTAAGTTCTTATACTCAATCATACA11.8181818181818181No Hit
AAATCCAACATCCACACTTAAGAAAACTAGATTAAAACTTAATCATTATT11.8181818181818181No Hit
GAATCCTGCAGGAACAGCTCAGCTTACTATTTTTTAAAAATAGAGCTTCT11.8181818181818181No Hit
GTTTTATATTTTTATTAAAACATTTTTAGAAAACAAACCACGAGATTATT11.8181818181818181No Hit
AGTGTAATGACGGAGATTGTGCATCAGCTTCCTCTCCTAATAAAATCACC11.8181818181818181No Hit
CATAAAGTACTGAAACATTGATACATAAAAAACTACCATAGCAAGAAATT11.8181818181818181No Hit
TAAACCGCAACCGCATCTTATAAGCCTAAGTAGTGTTTCCTTGTTAGAAG11.8181818181818181No Hit
CATTCACATGTAACATGCACACAAATCAAAACCCATTTTCCCAGAAGCAT11.8181818181818181No Hit
GGAAGCAACGCGCACGACAATACCGAGATAAAAGCTCCCGATCATGCCGA11.8181818181818181No Hit
CAAAGATTTTAGAGTTTACATTAGGCAAAAAAACATGAAAAAAACACAAG11.8181818181818181No Hit
GTGCAGCAATAAGATCAGACGATGAAGCCAAAAAGGGATTGGCTGCATAA11.8181818181818181No Hit
AAACTCCACTCTTAATAATCTGATCTTGAACTTGCTGACCAACTTCATGG11.8181818181818181No Hit
ATCGTGATGACGTTGGTAAATCGTCAGTATTTAGATCATTTTTCTATTTT11.8181818181818181No Hit
TTGATGTCATGTGTATGATTGAGTATAAGAACTTAAACCGCAACCTGATC11.8181818181818181No Hit
GCTTACCATTAGAGCTTCATTGGTTATACTCTGGGTAGATGGGACATATC11.8181818181818181No Hit
CACCTCGCTGCTTTAGCTCCGCTTTCGATCTGACGCAGCAAAATGGAAAG11.8181818181818181No Hit
TTGGTGTAGCCAAAACCCATATGAGTCTTTGGCTTTGTGTCTTCTAACAA11.8181818181818181No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)