FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n01_daplib_KyoAmpPoolHm-input_ac7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n01_daplib_KyoAmpPoolHm-input_ac7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCTCTTTTCCCCATTCGGTTTTATCTTCGTACCCGCAGCTAATAAC116.666666666666664No Hit
CGAATCACACACAAACTCACAACGTTTCTGTGAAGCTCGCCGTCACCGTC116.666666666666664No Hit
ATGAAAGCTTTGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTCGA116.666666666666664No Hit
AACAGTAGCTGAACTAGCGGGACTACCTACGTTTTTTGCGATTGTACAAC116.666666666666664No Hit
CCGATCTACTAATTCTTTGATTCCAGTTAGTATTAGTAAGAGGGATCTTG116.666666666666664No Hit
TTATCGAATTGGTTTCTCTATGAACTATATTGGTCATAAGTTCAGGGGAA116.666666666666664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)