FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n01_daplib_CviAmpPoolHm-input_ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n01_daplib_CviAmpPoolHm-input_ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATATGTTGAGAATATAATTAAGCATATGTTATAATGGTAAGATAGAAA12.941176470588235No Hit
GAGAAACTATGAGATGGGGAGAGCAGTTTGGGACGAGATGATCAAGAAAG12.941176470588235No Hit
TGACATCAAGTCATATTCGACTCCAAAACACTAACCAAGCTTCTTCTAGC12.941176470588235No Hit
AGATCAGTGAGAGACATTTGAAGCTTGCTTCTGTATCTGTATAGCCAAGA12.941176470588235No Hit
CCCGAAGATGAATTCACGAGAAATCTATTCCCAAGACCAGACCACAGCAC12.941176470588235No Hit
ATGAAAGCTTTGAGAAGCAATAATAAGGTTGGTTAGTGTTTTGGAGTCGA12.941176470588235No Hit
GGCCAAACTTGGCATGTGATACCTTTTCGGAAAGCCCAAAGACAGCCCTC12.941176470588235No Hit
AGTACTTAAAGGTCAAAATTTGGGGTCGACAAAAATTCAATGCAAATGTT12.941176470588235No Hit
ACGCAAGTGTAGCTATAAAACATGCATTGAGTGATGGTGTAGCATTCAAA12.941176470588235No Hit
AGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAA12.941176470588235No Hit
ATTTCGTGCCCCTTCGCTCAATGAGAAAATGGGTCAGATTCTAGATCGGA12.941176470588235No Hit
ACATTTCCTCTACAATTTTGTCTATTTCATTCTTCTAGTGGATTGGATAA12.941176470588235No Hit
CTCCACATTGGCCTGCAAATGCATAGAATTGGACTGCGCAAATATGTCTT12.941176470588235No Hit
ATAGTGGAAAAGTTGACAGATAAGTCACCCTTACTGCCACGCTACAGAAC12.941176470588235No Hit
CAGAAGTTGATGAGGTACACTGAGTTATACTTGAAAATCATAATTGGTAT12.941176470588235No Hit
TTAAACCATCACATCAGTTTTGGATCATATAACATCGAAGATGGAGAAGT12.941176470588235No Hit
ATTTTGATGAAGAAGCAGATATTCTCATTGCCGGTGACTTTATGGAGGAG12.941176470588235No Hit
TAAGCTTTCATGGTGTAGCCAAAGTCCGTATGAGTTTTTGGCTTTGTTTG12.941176470588235No Hit
GTAGAGCAAACGAAGCATGTCCAAACGAAAACCAACCTCTGGGGCTGCTA12.941176470588235No Hit
GATGTGGAACCAATTATCATAGCTAGACAAAACCCTGGATTTATATCCCT12.941176470588235No Hit
CGTGTGTTACCGCACGCGGTTGGCCTAAATCCGAGCCAAGGACGCCTGGA12.941176470588235No Hit
ACAACGTCACCACCAAAGTGCAGAGGATTCTAGCAAGTTAAAACTGGACA12.941176470588235No Hit
AAACAAACGTTAGAAACTTTGTTAGAGCTTTATGACTTGAGAGACTGTAT12.941176470588235No Hit
TTTAAGTTCTTATACTTAAACATACACATGACATCAAGTCATATTCGACT12.941176470588235No Hit
TCAGACACACGTAGTCATTTGTATATTGGAGTCTTAATACAGTAAGCATA12.941176470588235No Hit
TGGAGCTCAACCAACAAGAACAGCTAATGATATTACAAAGAAGGCTGGGA12.941176470588235No Hit
TTTTAAACACACACATAATCAGACTTTGATAATAATTCTTTTATGTTTCA12.941176470588235No Hit
AAAGTTTTGACAGTTCATGGTTCGGATGATACCGTTGTACCTGGGGAAGA12.941176470588235No Hit
GCTCGCACTGTTCTTTGTCTCCTCGAGCGCCAAATGAGACCTGGAGGCTC12.941176470588235No Hit
TATACAGGTGAAGGGTTCGAAGATATGCATCTTCAAGGTAGAAGATGGGC12.941176470588235No Hit
TGTTGGGAAAGGTTTCTTCATGAGAGAACCATTAGAGTTCTGCTTGTCGA12.941176470588235No Hit
ATAGCTCGACCAAGTAGACAAAAGTAAACGAGCTAGATGAATGAAACTAA12.941176470588235No Hit
TAGCGCAATTGGTGACTTCCTTCGCACTCATGGCAAAAGAAAGTGTCTTT12.941176470588235No Hit
GACCCATGTAAGAATGGCTAAGTAGTTATATATATCTTGGAGGTGATATG12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)