FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n01_daplib_An1AmpPoolHm-input_ac6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n01_daplib_An1AmpPoolHm-input_ac6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGGAAGAGGTCTCACAGCCTCAAATACGGTGTTTGGGAGATTCATAC12.2222222222222223No Hit
CTTCTCCAAAGTCCATATCAATAACATCTTAAAAACTACTCCGCTTCCCA12.2222222222222223No Hit
GTTGACTATTTGACAGAAGACAAGTGAAAGAACTGAATTGAAAAAAAAGA12.2222222222222223No Hit
AGGCCTTGGATAATGGTAGGCTATCCAGTATAAAATGCAACCCGCCAAAT12.2222222222222223No Hit
GATGAGTCTGATCTATTTAGCGGGCCATTGCAGATAGGACAGAAAGCTTC12.2222222222222223No Hit
AGAAGACACAAAGCCAAAGACTCATATGGACTTTGGCTACACCATGAAAG12.2222222222222223No Hit
AATACTCAGCCGTTGTGAGAGCAATACGAGGAGTCATGATACGTTCAATA12.2222222222222223No Hit
GAGATGCCCTTAGATGTTCTGGGCCGCACGCGCTCTACACTGATGAGATC12.2222222222222223No Hit
CTGCTGTTGCATAAATTATCATTTTAACGATCAACTTCTCCCATAATTAT12.2222222222222223No Hit
ACGGTTTTTGGTTCAGGACTTAGGATTCAGGGGTTAGGGTTTAGGGGTTT12.2222222222222223No Hit
CATGATTAACTCTTCCTCTGCTAAATTTGCTGGCTCACAATACGGTAGCT12.2222222222222223No Hit
AATCCGTGTGTTACTTCACACGGCTCGGTTACTCACCCCCTAAACACACG12.2222222222222223No Hit
GAAGCAAATGAAGAAGGAAGGACAGCTAGACGCAGCAGTTGAAGAAGATT12.2222222222222223No Hit
TACTCGACATTGACGTGATCTCTCATTTCTCTTTGTCTTTTTTCTCATTA12.2222222222222223No Hit
AACCTTGACCTTAGTCTTATCTAGTTTCTCCAACAGAGTATGTGCTTCAA12.2222222222222223No Hit
TTCCTCTCCAACATTCGTCATCGCCGCGTTCGTCGAGCACCAGTAACCAC12.2222222222222223No Hit
GTTAGTAATAGAAGACTACTTAACTAAATTCCCGACGGTGCATACTTGTG12.2222222222222223No Hit
TAGGGCAGAAAGAAATAATAGAAAAAGAGCTCCAGTATAAAGATCTTCAT12.2222222222222223No Hit
TTGCAGTTTAAGTTCTTATACTCAATCATACACATGAGATCAAGTCATAT12.2222222222222223No Hit
TTCTCGAATTTAAATAGCTTGGTCGAACCTGTTTCAAGCATTTGTCTGTC12.2222222222222223No Hit
CCTGAGGGAGAGCTTGGTGAGAATGGTGGATTGGGCCGTCTTATGATGGA12.2222222222222223No Hit
GATCCAGTACTCGATCCATTTACTCGACCAAGGGTGACCATCTCTACAAG12.2222222222222223No Hit
GTCAATAACAGTCTCACTTCCTACTTCAATACGCCCGATTTGGTTAGGGT12.2222222222222223No Hit
AAATGGTCAAGCATTTGGTTCGAGTAGAATTGCTGAATTTTTGGGGACAG12.2222222222222223No Hit
AGCTAACTCTTCTATTTCTCTCTTCTTGTGGTCAAAAGTTCTCTTCACCA12.2222222222222223No Hit
AACGACGAGAAAACACTCGAACGTGAGAGCAAGGGATCACCCAACGAATG12.2222222222222223No Hit
ATTGGTCTTGAGGTTTCTTCAGTCTCTAAGAATCCAACTTAATCCTTACT12.2222222222222223No Hit
TCGGTGTTGGGATATATAAGCCTATGTGGCCAAACACACACCGACCTTTT12.2222222222222223No Hit
AACTAAGGAGCCAAAGGTTGAGACTGTGACTCTTGGACCTTCTGTTCGTG12.2222222222222223No Hit
GTTTACGCAACAGGAGCAGAGATGGCTGATTCTCAAACCATAGATCCTAA12.2222222222222223No Hit
ACCAAAACACCACCAAACAGCATCGTAAAGTCTTAATAAAAGTATCAGAA12.2222222222222223No Hit
TGGCATCTGCGACTCTGCGTCTTCTGTTTGATCTGAACAATGATGTAGAT12.2222222222222223No Hit
CGCCCATGGGCATCGACACCTTGCGGCGAGGAACTGGAACGAGACGGGTA12.2222222222222223No Hit
TGGCTTTGTGTCTTCTAACAAGGAAACGCTACTTAGGCTTATAAGATGCG12.2222222222222223No Hit
ATTCTCGAAGTGATAAGAATTCAGATTTGCTATGATCCTATGTATCGTAA12.2222222222222223No Hit
CTTGGCAATTATCTATCCGAAGTGGAAGGTTAACCGGGATAAAATCCTGG12.2222222222222223No Hit
AGCAACAGACAACTTTAGTAAAGACAGATTACTAGGAGATGGAGGCTTTG12.2222222222222223No Hit
TGTGATTTTGTTACCGATTTGATTTGTGTTAGATGAGGAACGGATATTTA12.2222222222222223No Hit
AGCCAAAGGCGATATGAGTCTTTGGCTTTATGTCTTCTAACAAGGAAACA12.2222222222222223No Hit
GTTCAATGAATTGAGAAACATATAGGCCATTTCCTTTAGAAACAACGTCA12.2222222222222223No Hit
GAAGGTGCATAGTGAGAAGAGTAAGTCAAGAGACAGACTTGTTCAAAAAG12.2222222222222223No Hit
AACCGCAACCGGATCTTAGGGCGTAAGAATTGTATCCTTGTTAAAAGAAA12.2222222222222223No Hit
TCGAAAATCACTTGATGTCATGTGTAAGATTGAGAATAAGAATTTAAACC12.2222222222222223No Hit
ACAAACAGGACCATCATGGTTCGAACCAGGGATAGTCTCAGTGACCACTC12.2222222222222223No Hit
TGGAAAATGGTTCTTTTCCTACCTTTGTCAAAGATGCACATGAATAAGTA12.2222222222222223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)