FastQCFastQC Report
Fri 14 Jul 2023
HGWL2AFX5_n01_FaWRKY25_ampFa-B_ac2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWL2AFX5_n01_FaWRKY25_ampFa-B_ac2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13196327
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG7042135.336431872292949TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT409690.3104575993001689TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG224990.17049441105847105TruSeq Adapter, Index 6 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCGGGGGG145530.11028068643646068TruSeq Adapter, Index 6 (97% over 36bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG145000.10987905952921596TruSeq Adapter, Index 3 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)