FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10393544
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG215990220.781188784114445No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG443350.4265628740302634No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC408400.39293623041380304Illumina Single End PCR Primer 1 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151870.14611955267616128No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103970.1000332514106834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG333400.065.8434218
AGAGCAC435600.065.77083615
GCACGCG81200.065.7228718
CACGCGT43550.065.5724619
ACACGAG144700.065.2527920
AGCACAC334750.065.200617
CACGTAG130600.065.19749521
CACACGA144900.064.79951519
CGCGCGT53600.063.6589219
CACACGT185500.063.14387519
ACACGTA126350.062.48698420
GAGCACA400900.062.3855716
TAACTGA138250.062.302872
AAGAGCA501550.061.33150514
CACGCGA27050.060.67712819
ACGTAGA130200.060.64041522
ACTGAAG142150.059.631884
ACGAGAT155300.059.53816222
GGCACGC18100.059.35484717
GATCATC80100.058.9872710
Produced by FastQC (version 0.11.5)